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ZO1 Recombinant Rabbit Monoclonal Antibody [PSH07-09]

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Specification

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Catalog# HA722797

ZO1 Recombinant Rabbit Monoclonal Antibody [PSH07-09]

Application

  • WB

  • IF-Cell

  • mIHC

Reactivity

  • Human

  • Mouse

  • Green monkey

BSA and Azide free
Predicted reactivity

  • Cynomolgus monkey

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

ZO1 Recombinant Rabbit Monoclonal Antibody [PSH07-09]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ZO1 aa 1,401-1,748.

Species Reactivity

Human, Mouse, Green monkey (Predicted: Cynomolgus monkey)

Predicted species support after-sales service

Validated Applications

WB, IF-Cell, mIHC

Target Molecular Weight

Predicted band size: 195 kDa

Positive Control

A431 cell lysate, 293T cell lysate, HepG2 cell lysate, HeLa cell lysate, U-2 OS cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, COS-1 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, MCF7.

Conjugation

unconjugated

Clone Number

PSH07-09

同源性数据

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Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:500-1:2,000

  • mIHC

  • 1:100

Target

Function

Zonula occludens-1 ZO-1, also known as Tight junction protein-1 is a 220-kD peripheral membrane protein that is encoded by the TJP1 gene in humans. It belongs to the family of zonula occludens proteins (ZO-1, ZO-2, and ZO-3), which are tight junction-associated proteins and of which, ZO-1 is the first to be cloned. It was first isolated in 1986 by Stevenson and Goodenough using a monoclonal antibody raised in rodent liver to recognise a 225-kD polypeptide in whole liver homogenates and in tight junction-enriched membrane fractions. It has a role as a scaffold protein which cross-links and anchors Tight Junction (TJ) strand proteins, which are fibril-like structures within the lipid bilayer, to the actin cytoskeleton. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell–cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene.

Background References

1. Tsurudome Y et al. Decreased ZO1 expression causes loss of time-dependent tight junction function in the liver of ob/ob mice. Mol Biol Rep. 2022 Dec

2. Han F et al. GLTSCR1 coordinates alternative splicing and transcription elongation of ZO1 to regulate colorectal cancer progression. J Mol Cell Biol. 2022 Jun

Subcellular Location

Cell membrane, Cell junction, tight junction, gap junction, Cell projection, podosome.

UNIPROT

SwissProt: Q07157 Human

Synonyms

Tight junction protein 1 antibody

Tight junction protein ZO-1 antibody

Tight junction protein ZO1 antibody

TJP1 antibody

zo-1 antibody

Zo1 antibody

ZO1_HUMAN antibody

Zona occludens 1 antibody

Zona occludens 1 protein antibody

Zona occludens protein 1 antibody

Expand

Images

  • Western blot analysis of ZO1 on different lysates with Rabbit anti-ZO1 antibody (<a href="/products/HA722797" style="font-weight: bold;text-decoration: underline;">HA722797</a>) at 1/1,000 dilution.<br /><br />Lane 1: A431 cell lysate (20 µg/Lane)<br />Lane 2: 293T cell lysate (20 µg/Lane)<br />Lane 3: HepG2 cell lysate (20 µg/Lane)<br />Lane 4: HeLa cell lysate (20 µg/Lane)<br />Lane 5: U-2 OS cell lysate (20 µg/Lane)<br />Lane 6: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 7: C2C12 cell lysate (20 µg/Lane)<br />Lane 8: COS-1 cell lysate (20 µg/Lane)<br />Lane 9: Mouse testis tissue lysate (40 µg/Lane)<br />Lane 10: Rat testis tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 195 kDa<br />Observed band size: 250 kDa<br /><br />Exposure time: 3 minutes; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722797" style="font-weight: bold;text-decoration: underline;">HA722797</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ZO1 on different lysates with Rabbit anti-ZO1 antibody (HA722797) at 1/1,000 dilution.

    Lane 1: A431 cell lysate (20 µg/Lane)
    Lane 2: 293T cell lysate (20 µg/Lane)
    Lane 3: HepG2 cell lysate (20 µg/Lane)
    Lane 4: HeLa cell lysate (20 µg/Lane)
    Lane 5: U-2 OS cell lysate (20 µg/Lane)
    Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 7: C2C12 cell lysate (20 µg/Lane)
    Lane 8: COS-1 cell lysate (20 µg/Lane)
    Lane 9: Mouse testis tissue lysate (40 µg/Lane)
    Lane 10: Rat testis tissue lysate (40 µg/Lane)

    Predicted band size: 195 kDa
    Observed band size: 250 kDa

    Exposure time: 3 minutes; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722797) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of MCF7 cells labeling ZO1 with Rabbit anti-ZO1 antibody (<a href="/products/HA722797" style="font-weight: bold;text-decoration: underline;">HA722797</a>) at 1/2,000 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ZO1 antibody (<a href="/products/HA722797" style="font-weight: bold;text-decoration: underline;">HA722797</a>) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of MCF7 cells labeling ZO1 with Rabbit anti-ZO1 antibody (HA722797) at 1/2,000 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ZO1 antibody (HA722797) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation