Apolipoprotein CI / Apo-CI Recombinant Rabbit Monoclonal Antibody [JE36-30]
Catalog# HA722783
Apolipoprotein CI / Apo-CI Recombinant Rabbit Monoclonal Antibody [JE36-30]
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WB
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Human
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unconjugated
Overview
Product Name
Apolipoprotein CI / Apo-CI Recombinant Rabbit Monoclonal Antibody [JE36-30]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Apo-CI aa 1-83 / 83.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 9 kDa
Positive Control
HepG2 cell lysate, HepG2 treated with 300ng/mL BFA for 24 hours cell lysate.
Conjugation
unconjugated
Clone Number
JE36-30
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Apolipoprotein C-I is a protein component of lipoproteins that in humans is encoded by the APOC1 gene. The protein encoded by this gene is a member of the apolipoprotein C family. This gene is expressed primarily in the liver, and it is activated when monocytes differentiate into macrophages. Alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Background References
1. Hao X et al. Inhibition of APOC1 promotes the transformation of M2 into M1 macrophages via the ferroptosis pathway and enhances anti-PD1 immunotherapy in hepatocellular carcinoma based on single-cell RNA sequencing. Redox Biol. 2022 Oct
2. Ren L et al. Systematic pan-cancer analysis identifies APOC1 as an immunological biomarker which regulates macrophage polarization and promotes tumor metastasis. Pharmacol Res. 2022 Sep
Subcellular Location
Secreted.
UNIPROT
Synonyms
APO C1 antibody
Apo CI antibody
Apo-CIB antibody
Apo-CIB' antibody
APOC 1 antibody
ApoC I antibody
ApoC-IB antibody
ApoC-IB' antibody
APOC1 antibody
APOC1_HUMAN antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Apolipoprotein CI / Apo-CI on different lysates with Rabbit anti-Apolipoprotein CI / Apo-CI antibody (HA722783) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HepG2 treated with 300ng/mL BFA for 24 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 9 kDa
Observed band size: 9 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722783) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"