Phospho-STAT6 (Y641) Recombinant Rabbit Monoclonal Antibody [PSH06-98] (HA722776)
Catalog# HA722776
Rabbit Monoclonal to Phospho-STAT6 (Y641)
Tested for
-
WB
Reacts with
-
Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-STAT6 (Y641) on different lysates with Rabbit anti-Phospho-STAT6 (Y641) antibody (HA722776) at 1/1,000 dilution.
Lane 1: Daudi cell lysate
Lane 2: Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate
Lane 3: Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 94 kDa
Observed band size: 100 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722776) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-STAT6 (Y641) on different lysates with Rabbit anti-Phospho-STAT6 (Y641) antibody (HA722776) at 1/1,000 dilution.
Lane 1: Daudi cell lysate
Lane 2: Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate
Lane 3: Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 94 kDa
Observed band size: 100 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722776) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.