FRA1 Recombinant Rabbit Monoclonal Antibody [PSH06-95]
Catalog# HA722771
FRA1 Recombinant Rabbit Monoclonal Antibody [PSH06-95]
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WB
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Human
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unconjugated
Overview
Product Name
FRA1 Recombinant Rabbit Monoclonal Antibody [PSH06-95]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human FRA1 aa 1-271.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 29 kDa
Positive Control
HeLa starved for 24 hours then add 100ng/mL TPA for 4 hours cell lysate.
Conjugation
unconjugated
Clone Number
PSH06-95
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
Fos-related antigen 1 (FRA1) is a protein that in humans is encoded by the FOSL1 gene. The Fos gene family consists of 4 members: c-Fos, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation.
Background References
1. Manetsch P et al. PARP7-mediated ADP-ribosylation of FRA1 promotes cancer cell growth by repressing IRF1- and IRF3-dependent apoptosis. Proc Natl Acad Sci U S A. 2023 Dec
2. Jiang X et al. Expression and function of FRA1 protein in tumors. Mol Biol Rep. 2020 Jan
Subcellular Location
Nucleus.
UNIPROT
Synonyms
FOS L1 antibody
FOS like antigen 1 antibody
Fos related antigen 1 antibody
Fos-related antigen 1 antibody
FOSL 1 antibody
FOSL1 antibody
FOSL1 protein antibody
FOSL1_HUMAN antibody
FRA 1 antibody
FRA-1 antibody
Images
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Western blot analysis of FRA1 on different lysates with Rabbit anti-FRA1 antibody (HA722771) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa starved for 24 hours then add 100ng/mL TPA for 4 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 35-40 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722771) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"