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Phospho-IKB alpha (S32/S36) Recombinant Rabbit Monoclonal Antibody [PSH06-76] (HA722770)

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Catalog# HA722770

Rabbit Monoclonal to Phospho-IkB alpha (S32/S36)

Tested for

  • WB

  • IHC-P

  • IF-Cell

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho-IKB alpha (S32/S36) Recombinant Rabbit Monoclonal Antibody [PSH06-76] (HA722770)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding ser32/ser36 of human ikb alpha aa 1-50.

Host

Rabbit

Modification

Phospho

Modification Site

S32/S36

Positive Control

HeLa treated with 20ng/mL TNF-α and 100nM Calyculin A for 10 minutes cell lysate, NIH/3T3 treated with 20ng/mL TNF-α and 100nM Calyculin A for 10 minutes cell lysate, C6 treated with 100ng/mL Calyculin A for 1 hours cell lysate, human breast cancer tissue, HeLa cells treated with 20ng/mL TNF-α and 100nM Calyculin A for 10 minutes, NIH/3T3 cells treated with 20ng/mL TNF-α and 100nM Calyculin A for 10 minutes.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

PSH06-76

APPLICATION DILUTION

  • WB

  • 1:5,000

  • IHC-P

  • 1:200

  • IF-Cell

  • 1:100-1:1,000

PROPERTIES

Form

Liquid

Storage Condition

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 36 kDa

Isotype

IgG

TARGET

UNIPROT #

P25963

PROTEIN NAME

Phospho-IkB alpha (S32/S36)

SYNONYMS

I kappa B alpha antibody;I-kappa-B-alpha antibody;IkappaBalpha antibody;IkB-alpha antibody;IKBA antibody;IKBA_HUMAN antibody;IKBalpha antibody;MAD 3 antibody;MAD3 antibody;Major histocompatibility complex enhancer-binding protein MAD3 antibody;NF kappa B inhibitor alpha antibody;NF-kappa-B inhibitor alpha antibody;NFKBI antibody;NFKBIA antibody;Nuclear factor of kappa light chain gene enhancer in B cells antibody;Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

IκBα (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha; NFKBIA) is one member of a family of cellular proteins that function to inhibit the NF-κB transcription factor. IκBα inhibits NF-κB by masking the nuclear localization signals (NLS) of NF-κB proteins and keeping them sequestered in an inactive state in the cytoplasm. In addition, IκBα blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-κB's proper functioning.

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