Phospho-Met (Y1234/Y1235) Recombinant Rabbit Monoclonal Antibody [PSH06-90] (HA722765)
Catalog# HA722765
Rabbit Monoclonal to Phospho-Met (Y1234/Y1235)
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-Met (Y1234/Y1235) on different lysates with Rabbit anti-Phospho-Met (Y1234/Y1235) antibody (HA722765) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa starved for 16 hours then treated with 20ng/mL HGF for 10 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 156 kDa
Observed band size: 145 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722765) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-Met (Y1234/Y1235) on different lysates with Rabbit anti-Phospho-Met (Y1234/Y1235) antibody (HA722765) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa starved for 16 hours then treated with 20ng/mL HGF for 10 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 156 kDa
Observed band size: 145 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722765) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.