NEMP1 Recombinant Rabbit Monoclonal Antibody [PSH06-66] (HA722738)
Catalog# HA722738
Rabbit Monoclonal to NEMP1
Tested for
-
WB
-
IF-Cell
Reacts with
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of NEMP1 on different lysates with Rabbit anti-NEMP1 antibody (HA722738) at 1/1,000 dilution.
Lane 1: Mouse spleen tissue lysate (no heat)
Lane 2: Mouse skin tissue lysate (hot lysis)
Lane 3: Mouse placenta tissue lysate (70℃ heat)
Lane 4: Rat spleen tissue lysate (no heat)
Lane 5: Rat skin tissue lysate (hot lysis)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 40 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722738) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse spleen tissue lysate (no heat)
Lane 2: Mouse skin tissue lysate (hot lysis)
Lane 3: Mouse placenta tissue lysate (70℃ heat)
Lane 4: Rat spleen tissue lysate (no heat)
Lane 5: Rat skin tissue lysate (hot lysis)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 40 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722738) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.