CD68 Recombinant Rabbit Monoclonal Antibody [PSH06-65] (HA722737)
Catalog# HA722737
Rabbit Monoclonal to CD68
Tested for
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WB
-
IF-Cell
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IHC-P
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IF-Tissue
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IHC-Fr
Reacts with
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CD68 on different lysates with Rabbit anti-CD68 antibody (HA722737) at 1/5,000 dilution.
Lane 1: RAW264.7 cell lysate (20 µg/Lane)
Lane 2: M‑NFS‑60 cell lysate (20 µg/Lane)
Lane 3: J774A.1 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (negative) (20 µg/Lane)
Lane 5: Neuro-2a cell lysate (negative) (20 µg/Lane)
Lane 6: Rat spleen tissue lysate (no heat) (40 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 35 kDa
Observed band size: 90-200 kDa
Exposure time: 12 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722737) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD68 on different lysates with Rabbit anti-CD68 antibody (HA722737) at 1/5,000 dilution.
Lane 1: RAW264.7 cell lysate (20 µg/Lane)
Lane 2: M‑NFS‑60 cell lysate (20 µg/Lane)
Lane 3: J774A.1 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (negative) (20 µg/Lane)
Lane 5: Neuro-2a cell lysate (negative) (20 µg/Lane)
Lane 6: Rat spleen tissue lysate (no heat) (40 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 35 kDa
Observed band size: 90-200 kDa
Exposure time: 12 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722737) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.