ATF6 Recombinant Rabbit Monoclonal Antibody [PSH06-59] (HA722722)
Catalog# HA722722
Rabbit Monoclonal to ATF6
Tested for
-
WB
-
IHC-P
-
ChIP
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of ATF6 on different lysates with Rabbit anti-ATF6 antibody (HA722722) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: HUVEC cell lysate
Lane 4: 293T cell lysate
Lane 5: HepG2 cell lysate
Lane 6: RAW264.7 cell lysate
Lane 7: PC-12 cell lysate
Lane 8: Mouse pancreas tissue lysate
Lane 9: Mouse kidney tissue lysate
Lane 10: Rat pancreas tissue lysate
Lane 11: Rat kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 75 kDa
Observed band size: 100-110 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722722) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: HUVEC cell lysate
Lane 4: 293T cell lysate
Lane 5: HepG2 cell lysate
Lane 6: RAW264.7 cell lysate
Lane 7: PC-12 cell lysate
Lane 8: Mouse pancreas tissue lysate
Lane 9: Mouse kidney tissue lysate
Lane 10: Rat pancreas tissue lysate
Lane 11: Rat kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 75 kDa
Observed band size: 100-110 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722722) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.