HES5 Recombinant Rabbit Monoclonal Antibody [JE35-96] (HA722699)
Catalog# HA722699
Rabbit Monoclonal to HES5
Tested for
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WB
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IHC-P
Reacts with
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of HES5 on different lysates with Rabbit anti-HES5 antibody (HA722699) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: U-87 MG cell lysate
Lane 3: C6 cell lysate
Lane 4: Mouse kidney tissue lysate
Lane 5: Rat liver tissue lysate
Lane 6: Rat kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722699) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HEK-293 cell lysate
Lane 2: U-87 MG cell lysate
Lane 3: C6 cell lysate
Lane 4: Mouse kidney tissue lysate
Lane 5: Rat liver tissue lysate
Lane 6: Rat kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722699) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.