Lin28B Recombinant Rabbit Monoclonal Antibody [JE04-80] (HA722688)
Catalog# HA722688
Rabbit Monoclonal to Lin28B
Tested for
-
WB
-
IF-Cell
-
FC
-
IP
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Lin28B on different lysates with Rabbit anti-Lin28B antibody (HA722688) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: MCF7 cell lysate (negative)
Lane 3: JAR cell lysate
Lane 4: K-562 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 30 kDa
Observed band size: 35 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722688) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Lin28B on different lysates with Rabbit anti-Lin28B antibody (HA722688) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: MCF7 cell lysate (negative)
Lane 3: JAR cell lysate
Lane 4: K-562 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 30 kDa
Observed band size: 35 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722688) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.