Torsin A / DYT1 Recombinant Rabbit Monoclonal Antibody [JE01-51]
Catalog# HA722686
Torsin A / DYT1 Recombinant Rabbit Monoclonal Antibody [JE01-51]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Torsin A / DYT1 Recombinant Rabbit Monoclonal Antibody [JE01-51]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Torsin A / DYT1 aa 283-332 / 332.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 38 kDa
Positive Control
HepG2 cell lysate, 293T cell lysate, A549 cell lysate, SW480 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse brain tissue lysate.
Conjugation
unconjugated
Clone Number
JE01-51
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | IF-Cell | |
|---|---|---|---|
| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Torsin-1A (TorA) also known as dystonia 1 protein (DYT1) is a protein that in humans is encoded by the TOR1A gene (also known as DQ2 or DYT1). TorA localizes to the endoplasmic reticulum and contiguous perinuclear space, where its ATPase activity is activated by either LULL1 or LAP1, respectively. The protein encoded by this gene is a member of the AAA family of adenosine triphosphatases (ATPases), is related to the Clp protease/heat shock family.
Background References
1. Fan Y et al. DYT-TOR1A dystonia: an update on pathogenesis and treatment. Front Neurosci. 2023 Aug
2. Pocratsky AM et al. Pathophysiology of Dyt1-Tor1a dystonia in mice is mediated by spinal neural circuit dysfunction. Sci Transl Med. 2023 May
Subcellular Location
Endoplasmic reticulum lumen, Nucleus membrane, Cell projection, growth cone, Cytoplasmic vesicle membrane, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle, Cytoplasm, cytoskeleton.
UNIPROT
Synonyms
DQ2 antibody
Dystonia 1 antibody
Dystonia 1 protein antibody
Dystonia 1 torsion antibody
Dyt1 antibody
TOR1A antibody
TOR1A_HUMAN antibody
Torsin 1A antibody
Torsin A antibody
Torsin family 1 member A antibody
ExpandImages
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Western blot analysis of Torsin A / DYT1 on different lysates with Rabbit anti-Torsin A / DYT1 antibody (HA722686) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: SW480 cell lysate (20 µg/Lane)
Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
Lane 6: C6 cell lysate (20 µg/Lane)
Lane 7: Mouse brain tissue lysate (40 µg/Lane)
Predicted band size: 38 kDa
Observed band size: 35 kDa
Exposure time: 1 minute 2 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722686) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"