VTI1B Recombinant Rabbit Monoclonal Antibody [PSH06-40] (HA722675)
Catalog# HA722675
Rabbit Monoclonal to VTI1B
Tested for
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WB
-
IF-Cell
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IHC-P
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FC
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of VTI1B on different lysates with Rabbit anti-VTI1B antibody (HA722675) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: U-2 OS cell lysate (20 µg/Lane)
Lane 4: U-87 MG cell lysate (20 µg/Lane)
Lane 5: SH-SY5Y cell lysate (20 µg/Lane)
Lane 6: C2C12 cell lysate (20 µg/Lane)
Lane 7: PC-12 cell lysate (20 µg/Lane)
Lane 8: C6 cell lysate (20 µg/Lane)
Lane 9: Rat brain tissue lysate (40 µg/Lane)
Lane 10: Rat kidney tissue lysate (40 µg/Lane)
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722675) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: U-2 OS cell lysate (20 µg/Lane)
Lane 4: U-87 MG cell lysate (20 µg/Lane)
Lane 5: SH-SY5Y cell lysate (20 µg/Lane)
Lane 6: C2C12 cell lysate (20 µg/Lane)
Lane 7: PC-12 cell lysate (20 µg/Lane)
Lane 8: C6 cell lysate (20 µg/Lane)
Lane 9: Rat brain tissue lysate (40 µg/Lane)
Lane 10: Rat kidney tissue lysate (40 µg/Lane)
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722675) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.