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Phospho-p38 alpha (T180 + Y182) Recombinant Rabbit Monoclonal Antibody [PSH06-35] (HA722669)

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Catalog# HA722669

Rabbit Monoclonal to Phospho-p38 alpha (T180/Y182)

Tested for

  • WB

  • IF-Cell

  • FC

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free

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Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho-p38 alpha (T180 + Y182) Recombinant Rabbit Monoclonal Antibody [PSH06-35] (HA722669)

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding thr180/tyr182 of human p38 alpha / mapk14.

Specificity

Phospho-p38 map kinase (thr180+tyr182) [psh06-35] rabbit mab detects endogenous levels of p38 alpha only when dually phosphorylated at thr180 and tyr182. it will also cross-react with the phosphorylated forms of either p38 beta, p38 gamma or p38 delta.

Host

Rabbit

Modification

Phospho

Modification Site

T180/Y182

Positive Control

Jurkat cell lysate, Jurkat treated with UV for 1 hour cell lysate, NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes cell lysate, C6 treated with 25μg/mL Anisomycin for 30 minutes cell lysate, PC-12 cell lysate, PC-12 treated with 25μg/mL Anisomycin for 30 minutes cell lysate, Jurkat cells treated with UV for 1 hour, NIH/3T3 cells treated with 25μg/mL Anisomycin for 30 minutes.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

PSH06-35

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:50-1:100

  • FC

  • 1:1,000

PROPERTIES

Form

Liquid

Storage Condition

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 41 kDa

Isotype

IgG

TARGET

UNIPROT #

Q16539

PROTEIN NAME

Phospho-p38 alpha (T180/Y182)

SYNONYMS

CSAID Binding Protein 1 antibody;CSAID binding protein antibody;CSAID-binding protein antibody;Csaids binding protein antibody;CSBP 1 antibody;CSBP 2 antibody;CSBP antibody;CSBP1 antibody;CSBP2 antibody;CSPB1 antibody;Cytokine suppressive anti-inflammatory drug-binding protein antibody;EXIP antibody;MAP kinase 14 antibody;MAP kinase MXI2 antibody;MAP kinase p38 alpha antibody;MAPK 14 antibody;MAPK14 antibody;MAX interacting protein 2 antibody;MAX-interacting protein 2 antibody;Mitogen Activated Protein Kinase 14 antibody;Mitogen activated protein kinase p38 alpha antibody;Mitogen-activated protein kinase 14 antibody;Mitogen-activated protein kinase p38 alpha antibody;MK14_HUMAN antibody;Mxi 2 antibody;MXI2 antibody;p38 ALPHA antibody;p38 antibody;p38 MAP kinase antibody;p38 MAPK antibody;p38 mitogen activated protein kinase antibody;p38ALPHA antibody;p38alpha Exip antibody;PRKM14 antibody;PRKM15 antibody;RK antibody;SAPK2A antibody;Stress-activated protein kinase 2a antibody

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

MAP (mitogen-activated protein) kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. p38α, p38β and p38γ, also known as MAPK14, MAPK11 and MAPK12, respectively, each contain one protein kinase domain and belong to the MAP kinase family. Expressed in different areas throughout the body with common expression patterns in heart, p38 proteins use magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Via their catalytic activity, p38α, p38β and p38γ are involved in a variety of events throughout the cell, including signal transduction pathways, cytokine production and cell proliferation and differentiation. The p38 proteins are subject to phosphoryation on Thr and Tyr residues, an event which is thought to activate the phosphorylated protein.

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