Bcl G/BCL2L14 Recombinant Rabbit Monoclonal Antibody [JE77-24]
Catalog# HA722583
Bcl G/BCL2L14 Recombinant Rabbit Monoclonal Antibody [JE77-24]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Bcl G/BCL2L14 Recombinant Rabbit Monoclonal Antibody [JE77-24]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Bcl G/BCL2L14 aa 1-250 / 327.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 37 kDa
Positive Control
HeLa cell lysate, K-562 cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate.
Conjugation
unconjugated
Clone Number
JE77-24
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Apoptosis facilitator Bcl-2-like protein 14 is a protein that in humans is encoded by the BCL2L14 gene. The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Overexpression of this gene has been shown to induce apoptosis in cells. Four alternatively spliced transcript variants, which encode three distinct isoforms, have been reported for this gene.
Background References
1. Lee S et al. Landscape analysis of adjacent gene rearrangements reveals BCL2L14-ETV6 gene fusions in more aggressive triple-negative breast cancer. Proc Natl Acad Sci U S A. 2020 May
2. Yao X et al. Upregulation of miR-496 decreases cerebral ischemia/reperfusion injury by negatively regulating BCL2L14. Neurosci Lett. 2019 Mar
Subcellular Location
Cytoplasm.
Synonyms
Apoptosis facilitator Bcl 2 like protein 14 antibody
Apoptosis regulator BCL G antibody
Bcl2 L 14 antibody
BCL2 like 14 (apoptosis facilitator) antibody
BCL2 like 14 antibody
BCL2L14 antibody
BCLG antibody
testicular tissue protein Li 26 antibody
Images
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Western blot analysis of Bcl G/BCL2L14 on different lysates with Rabbit anti-Bcl G/BCL2L14 antibody (HA722583) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: C6 cell lysate
Lane 7: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 45 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722583) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"