PLSCR3 Recombinant Rabbit Monoclonal Antibody [JE63-89]
Catalog# HA722577
PLSCR3 Recombinant Rabbit Monoclonal Antibody [JE63-89]
-
WB
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
PLSCR3 Recombinant Rabbit Monoclonal Antibody [JE63-89]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human PLSCR3 aa 1-50 / 295.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 32 kDa
Positive Control
HeLa cell lysate, A549 cell lysate, HepG2 cell lysate, Mouse brain tissue lysate, Mouse kidney tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
JE63-89
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Phospholipid scramblase 3 is an enzyme that in humans is encoded by the PLSCR3 gene[5][6] (abbreviated to PLS3 in this section). Like the other phospholipid scramblase family members (PLS1, PLS2, PLS4), PLS3 is a type II plasma membrane protein that is rich in proline and integral in apoptosis, or programmed cell death. The regulation of apoptosis is critical for both cell development and tissue homeostasis. Although phospholipid scramblase is thought to exist in all eukaryotic cells, PLS3 is a protein that is novel to the mitochondria. This is very important because mitochondria are central in the apoptotic cell pathway. This newly found member of the scramblase family is "responsible for phospholipid translocation between two lipid compartments", the inner mitochondrial membrane and the outer membrane. Further experimental evidence suggests that the mechanism and effectors of PLS3's enzymatic activity are rather nuanced.
Background References
1. Palanirajan SK et al. Phospholipid scramblase 3: a latent mediator connecting mitochondria and heavy metal apoptosis. Cell Biochem Biophys. 2023 Sep
2. Luévano-Martínez LA et al. Topological characterization of the mitochondrial phospholipid scramblase 3. FEBS Lett. 2017 Dec
Subcellular Location
Mitochondrion membrane, Mitochondrion inner membrane, Nucleus.
Synonyms
Ca(2+)-dependent phospholipid scramblase 3 antibody
Phospholipid scramblase 3 antibody
PL scramblase 3 antibody
PLS3 antibody
PLS3_HUMAN antibody
Plscr3 antibody
Images
-
Western blot analysis of PLSCR3 on different lysates with Rabbit anti-PLSCR3 antibody (HA722577) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Mouse kidney tissue lysate
Lane 6: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722577) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"