Phospho-MCM2 (S27) Recombinant Rabbit Monoclonal Antibody [JE63-40] (HA722574)
Catalog# HA722574
Rabbit Monoclonal to Phospho-MCM2 (S27)
Tested for
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WB
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IF-Cell
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IHC-P
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IP
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-MCM2 (S27) on different lysates with Rabbit anti-Phospho-MCM2 (S27) antibody (HA722574) at 1/1,000 dilution and pan MCM2 antibody (HA721553) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: Mouse thymus tissue lysate (40 µg/Lane)
Lane 5: Rat thymus tissue lysate (40 µg/Lane)
Lane 6: HeLa cell lysate, the membrane treated with λpp for 1 hour (20 µg/Lane)
Predicted band size: 102 kDa
Observed band size: 140 kDa
Exposure time: 17 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722574) at 1/1,000 dilution and pan MCM2 antibody (HA721553) at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-MCM2 (S27) on different lysates with Rabbit anti-Phospho-MCM2 (S27) antibody (HA722574) at 1/1,000 dilution and pan MCM2 antibody (HA721553) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: Mouse thymus tissue lysate (40 µg/Lane)
Lane 5: Rat thymus tissue lysate (40 µg/Lane)
Lane 6: HeLa cell lysate, the membrane treated with λpp for 1 hour (20 µg/Lane)
Predicted band size: 102 kDa
Observed band size: 140 kDa
Exposure time: 17 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722574) at 1/1,000 dilution and pan MCM2 antibody (HA721553) at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.