Nck-2 Recombinant Rabbit Monoclonal Antibody [JE63-36] (HA722573)
Catalog# HA722573
Rabbit Monoclonal to Nck-2
Tested for
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WB
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IF-Cell
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IHC-P
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IF-Tissue
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Nck-2 on different lysates with Rabbit anti-Nck-2 antibody (HA722573) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: SK-MEL-28 cell lysate
Lane 3: HDLM-2 cell lysate
Lane 4: Caco-2 cell lysate
Lane 5: THP-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 46 kDa
Exposure time: 1 minute 34 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722573) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate
Lane 2: SK-MEL-28 cell lysate
Lane 3: HDLM-2 cell lysate
Lane 4: Caco-2 cell lysate
Lane 5: THP-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 46 kDa
Exposure time: 1 minute 34 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722573) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.