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EYA2 Recombinant Rabbit Monoclonal Antibody [JE63-31]

Usd: 385

Specification

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Catalog# HA722572

EYA2 Recombinant Rabbit Monoclonal Antibody [JE63-31]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

EYA2 Recombinant Rabbit Monoclonal Antibody [JE63-31]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human EYA2 aa 1-50 / 538.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 59 kDa

Positive Control

HeLa cell lysate, 293T cell lysate, A549 cell lysate.

Conjugation

unconjugated

Clone Number

JE63-31

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Eyes absent homolog 2 is a protein that in humans is encoded by the EYA2 gene. This gene encodes a member of the eyes absent (EYA) subfamily of proteins. The encoded protein may be post-translationally modified and may play a role in eye development. A similar protein in mice can act as a transcriptional activator. Five transcript variants encoding three distinct isoforms have been identified for this gene.

Background References

1. Wolin AR et al. EYA2 tyrosine phosphatase inhibition reduces MYC and prevents medulloblastoma progression. Neuro Oncol. 2023 Dec

2. Liu ZK et al. EYA2 suppresses the progression of hepatocellular carcinoma via SOCS3-mediated blockade of JAK/STAT signaling. Mol Cancer. 2021 May

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: O00167 Human

Synonyms

EAB 1 antibody

EAB1 antibody

EYA 2 antibody

EYA transcriptional coactivator and phosphatase 2 antibody

EYA2 antibody

EYA2_HUMAN antibody

eyes absent 2 homolog (Drosophila) antibody

Eyes absent homolog 2 (Drosophila) antibody

Eyes absent homolog 2 antibody

Translation of this uORF probably lowers the translation efficiency of EYA2 antibody

Images

  • Western blot analysis of EYA2 on different lysates with Rabbit anti-EYA2 antibody (<a href="/products/HA722572" style="font-weight: bold;text-decoration: underline;">HA722572</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: 293T cell lysate<br />Lane 3: A549 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 59 kDa<br />Observed band size: 56 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722572" style="font-weight: bold;text-decoration: underline;">HA722572</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of EYA2 on different lysates with Rabbit anti-EYA2 antibody (HA722572) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: 293T cell lysate
    Lane 3: A549 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 59 kDa
    Observed band size: 56 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722572) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation