SHC Recombinant Rabbit Monoclonal Antibody [JE57-45]
Catalog# HA722564
SHC Recombinant Rabbit Monoclonal Antibody [JE57-45]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SHC Recombinant Rabbit Monoclonal Antibody [JE57-45]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human SHC aa 501-550 / 583.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 63 kDa
Positive Control
Jurkat cell lysate, HepG2 cell lysate, A431 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, human lung cancer tissue.
Conjugation
unconjugated
Clone Number
JE57-45
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
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IHC-P
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1:1,000
Target
Function
Overexpression of SHC proteins are associated with cancer mitogenesis, carcinogenesis and metastasis. The SHC and its adaptor proteins transmit signaling of the cell surface receptors such as EGFR, erbV-2 and insulin receptors. p52SHC and p46SHC activate the Ras-ERK pathway. p66SHC inhibits ERK1/2 activity and antagonize mitogenic and survival abilities of T-lymphoma Jurkat cell lines. A rise in p66SHC promotes stress induced apoptosis. p66SHC functionally is also involved in regulating oxidative and stress- induced apoptosis – mediating steroid action through the redox signaling pathway. P52SHC and p66SHC have been found in steroid hormone-regulated cancer and metastasizes.
Background References
1. Jelinek HF et al. Human SHC-transforming protein 1 and its isoforms p66shc: A novel marker for prediabetes. J Diabetes Investig. 2021 Oct
2. Huang C et al. The longevity protein p66Shc is required for neonatal heart regeneration. J Mol Cell Cardiol. 2023 Apr
Subcellular Location
Cytoplasm, Cell junction, focal adhesion; Mitochondrion matrix; Mitochondrion.
Synonyms
FLJ26504 antibody
p66 antibody
p66SHC antibody
SH2 domain protein C1 antibody
SHC (Src homology 2 domain containing) transforming protein 1 antibody
SHC 1 antibody
SHC A antibody
SHC adaptor protein 1 antibody
Shc antibody
SHC transforming protein 1 antibody
ExpandImages
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Western blot analysis of SHC on different lysates with Rabbit anti-SHC antibody (HA722564) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A431 cell lysate
Lane 4: HeLa cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: RAW264.7 cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 63 kDa
Observed band size: 66/52/46 kDa
Exposure time: 1 minute 50 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722564) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-SHC antibody (HA722564) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722564) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"