CXCL5 Recombinant Rabbit Monoclonal Antibody [JE53-78] (HA722561)
Catalog# HA722561
Rabbit Monoclonal to CXCL5
Tested for
-
WB
-
IF-Cell
-
FC
-
IP
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of CXCL5 on different lysates with Rabbit anti-CXCL5 antibody (HA722561) at 1/1,000 dilution.
Lane 1: A549 cell lysate
Lane 2: A549 starved overnight treated with 10ng/mL TNF-alpha and 10nM PMA for 24 hours then add 300ng/mL BFA for 3 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 12 kDa
Observed band size: 8 kDa
Exposure time: 16 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722561) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CXCL5 on different lysates with Rabbit anti-CXCL5 antibody (HA722561) at 1/1,000 dilution.
Lane 1: A549 cell lysate
Lane 2: A549 starved overnight treated with 10ng/mL TNF-alpha and 10nM PMA for 24 hours then add 300ng/mL BFA for 3 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 12 kDa
Observed band size: 8 kDa
Exposure time: 16 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722561) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.