Desmoplakin Recombinant Rabbit Monoclonal Antibody [JE51-43] (HA722560)
Catalog# HA722560
Rabbit Monoclonal to Desmoplakin
Tested for
-
WB
-
IHC-P
-
IF-Tissue
Reacts with
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Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Desmoplakin on different lysates with Rabbit anti-Desmoplakin antibody (HA722560) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: HeLa cell lysate
Lane 3: BxPC-3 cell lysate
Lane 4: Rat skin tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 332 kDa
Observed band size: 332/260 kDa
Exposure time: 3 minutes; ECL: K1801;
3-8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722560) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate
Lane 2: HeLa cell lysate
Lane 3: BxPC-3 cell lysate
Lane 4: Rat skin tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 332 kDa
Observed band size: 332/260 kDa
Exposure time: 3 minutes; ECL: K1801;
3-8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722560) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.