PITX3 Recombinant Rabbit Monoclonal Antibody [JE41-12]
Catalog# HA722554
PITX3 Recombinant Rabbit Monoclonal Antibody [JE41-12]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PITX3 Recombinant Rabbit Monoclonal Antibody [JE41-12]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human PITX3 aa 1-50 / 302.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 32 kDa
Positive Control
HEK-293 cell lysate, SH-SY5Y cell lysate, C6 cell lysate, HeLa cell lysate, Mouse brain tissue lysate, Mouse lung tissue lysate, Mouse spleen tissue lysate, Rat brain tissue lysate, Rat lung tissue lysate, Rat spleen tissue lysate.
Conjugation
unconjugated
Clone Number
JE41-12
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Pituitary homeobox 3 is a protein that in humans is encoded by the PITX3 gene. This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family act as transcription factors. This protein is involved in lens formation during eye development, and the specification and terminal differentiation of mesencephalic dopamine neurons in the substantia nigra compacta that are lost in Parkinson's disease. Mutations of this gene have been associated with anterior segment mesenchymal dysgenesis (ASMD) and congenital cataracts.
Background References
1. Wang X et al. The Crucial Roles of Pitx3 in Midbrain Dopaminergic Neuron Development and Parkinson\'s Disease-Associated Neurodegeneration. Int J Mol Sci. 2023 May
2. Chen X et al. Pitx3 deficiency promotes age-dependent alterations in striatal medium spiny neurons. Front Aging Neurosci. 2022 Sep
Subcellular Location
Nucleus.
Synonyms
Homeobox protein PITX 3 antibody
Homeobox protein PITX3 antibody
MGC12766 antibody
Paired like homeodomain transcription factor 3 antibody
Paired-like homeodomain transcription factor 3 antibody
Pituitary homeobox 3 antibody
PITX 3 antibody
Pitx3 antibody
PITX3_HUMAN antibody
PTX 3 antibody
ExpandImages
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Western blot analysis of PITX3 on different lysates with Rabbit anti-PITX3 antibody (HA722554) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: SH-SY5Y cell lysate (20 µg/Lane)
Lane 3: C6 cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (20 µg/Lane)
Lane 5: Mouse brain tissue lysate (40 µg/Lane)
Lane 6: Mouse lung tissue lysate (40 µg/Lane)
Lane 7: Mouse spleen tissue lysate (40 µg/Lane)
Lane 8: Rat brain tissue lysate (40 µg/Lane)
Lane 9: Rat lung tissue lysate (40 µg/Lane)
Lane 10: Rat spleen tissue lysate (40 µg/Lane)
Predicted band size: 32 kDa
Observed band size: 35 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722554) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"