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Engrailed 1 Recombinant Rabbit Monoclonal Antibody [JE37-39]

Usd: 385

Specification

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Catalog# HA722546

Engrailed 1 Recombinant Rabbit Monoclonal Antibody [JE37-39]

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Engrailed 1 Recombinant Rabbit Monoclonal Antibody [JE37-39]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Engrailed 1 aa 343-392 / 392.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 40 kDa

Positive Control

Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

JE37-39

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

Homeobox protein engrailed-1 is a protein that in humans is encoded by the EN1 gene. Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the engrailed (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system and the limbs.

Background References

1. Mascharak S et al. Preventing Engrailed-1 activation in fibroblasts yields wound regeneration without scarring. Science. 2021 Apr

2. Xu J et al. Engrailed-1 Promotes Pancreatic Cancer Metastasis. Adv Sci (Weinh). 2024 Feb

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q05925 Human

SwissProt: P09065 Mouse

Entrez Gene: 685360 Rat

Synonyms

En 1 antibody

EN1 antibody

Engrailed 1 antibody

Engrailed antibody

Engrailed homeobox 1 antibody

Engrailed homolog 1 antibody

HME1_HUMAN antibody

Homeobox protein en-1 antibody

Homeobox protein engrailed 1 antibody

Homeobox protein engrailed-1 antibody

Expand

Images

  • Western blot analysis of Engrailed 1 on different lysates with Rabbit anti-Engrailed 1 antibody (<a href="/products/HA722546" style="font-weight: bold;text-decoration: underline;">HA722546</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human brain tissue lysate (20 µg/Lane)<br />Lane 2: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 3: Rat brain tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 40 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722546" style="font-weight: bold;text-decoration: underline;">HA722546</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Engrailed 1 on different lysates with Rabbit anti-Engrailed 1 antibody (HA722546) at 1/1,000 dilution.

    Lane 1: Human brain tissue lysate (20 µg/Lane)
    Lane 2: Mouse brain tissue lysate (20 µg/Lane)
    Lane 3: Rat brain tissue lysate (20 µg/Lane)

    Predicted band size: 40 kDa
    Observed band size: 50 kDa

    Exposure time: 10 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722546) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Engrailed 1 was immunoprecipitated from 0.2 mg rat brain tissue lysate with <a href="/products/HA722546" style="font-weight: bold;text-decoration: underline;">HA722546</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722546" style="font-weight: bold;text-decoration: underline;">HA722546</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: Rat brain tissue lysate (input)<br />Lane 2: <a href="/products/HA722546" style="font-weight: bold;text-decoration: underline;">HA722546</a> IP in rat brain tissue lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722546" style="font-weight: bold;text-decoration: underline;">HA722546</a> in rat brain tissue lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 50 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    Engrailed 1 was immunoprecipitated from 0.2 mg rat brain tissue lysate with HA722546 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722546 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: Rat brain tissue lysate (input)
    Lane 2: HA722546 IP in rat brain tissue lysate
    Lane 3: Rabbit IgG instead of HA722546 in rat brain tissue lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 50 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"