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Troponin I fast skeletal muscle Recombinant Rabbit Monoclonal Antibody [JE35-67]

Usd: 385

Specification

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Catalog# HA722542

Troponin I fast skeletal muscle Recombinant Rabbit Monoclonal Antibody [JE35-67]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Troponin I fast skeletal muscle Recombinant Rabbit Monoclonal Antibody [JE35-67]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Troponin I fast skeletal muscle aa 133-182 / 182.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 21 kDa

Positive Control

Rat skeletal muscle tissue lysates, human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue.

Conjugation

unconjugated

Clone Number

JE35-67

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

Troponin I, fast skeletal muscle is a protein that in humans is encoded by the TNNI2 gene. The TNNI2 gene is located at 11p15.5 in the human chromosomal genome, encoding the fast twitch skeletal muscle troponin I (fsTnI). fsTnI is a 21.3 kDa protein consisting of 182 amino acids including the first methionine with an isoelectric point (pI) of 8.74. It is the inhibitory subunit of the troponin complex in fast twitch skeletal muscle fibers.

Background References

1. Li Y et al. A TNNI2 variant c.525G>T causes distal arthrogryposis in a Chinese family. Mol Genet Genomic Med. 2022 Dec

2. Liu D et al. Chimeric RNA TNNI2-ACTA1-V1 Regulates Cell Proliferation by Regulating the Expression of NCOA3. Front Vet Sci. 2022 Jul

Subcellular Location

Cytosol, nucleus.

UNIPROT

SwissProt: P48788 Human

SwissProt: P13412 Mouse

SwissProt: P27768 Rat

Synonyms

AMCD 2B antibody

AMCD2B antibody

DA 2B antibody

DA2B antibody

fast skeletal muscle antibody

Fast twitch skeletal muscle troponin I antibody

fast-twitch isoform antibody

FSSV antibody

fsTnI antibody

TNNI 2 antibody

Expand

Images

  • Western blot analysis of Troponin I fast skeletal muscle on rat skeletal muscle tissue lysates with Rabbit anti-Troponin I fast skeletal muscle antibody (<a href="/products/HA722542" style="font-weight: bold;text-decoration: underline;">HA722542</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 21 kDa<br />Observed band size: 21 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722542" style="font-weight: bold;text-decoration: underline;">HA722542</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Troponin I fast skeletal muscle on rat skeletal muscle tissue lysates with Rabbit anti-Troponin I fast skeletal muscle antibody (HA722542) at 1/1,000 dilution.

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722542) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Troponin I fast skeletal muscle on different lysates with Rabbit anti-Troponin I fast skeletal muscle antibody (<a href="/products/HA722542" style="font-weight: bold;text-decoration: underline;">HA722542</a>) at 1/1,000 dilution.<br /><br />Lane 1: 293T-NT cell lysate<br />Lane 2: 293T-OE-TNNI2 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Exposure time: 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722542" style="font-weight: bold;text-decoration: underline;">HA722542</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Troponin I fast skeletal muscle on different lysates with Rabbit anti-Troponin I fast skeletal muscle antibody (HA722542) at 1/1,000 dilution.

    Lane 1: 293T-NT cell lysate
    Lane 2: 293T-OE-TNNI2 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Exposure time: 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722542) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Troponin I fast skeletal muscle antibody (<a href="/products/HA722542" style="font-weight: bold;text-decoration: underline;">HA722542</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722542" style="font-weight: bold;text-decoration: underline;">HA722542</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Troponin I fast skeletal muscle antibody (HA722542) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722542) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Troponin I fast skeletal muscle antibody (<a href="/products/HA722542" style="font-weight: bold;text-decoration: underline;">HA722542</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722542" style="font-weight: bold;text-decoration: underline;">HA722542</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Troponin I fast skeletal muscle antibody (HA722542) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722542) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Troponin I fast skeletal muscle antibody (<a href="/products/HA722542" style="font-weight: bold;text-decoration: underline;">HA722542</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722542" style="font-weight: bold;text-decoration: underline;">HA722542</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Troponin I fast skeletal muscle antibody (HA722542) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722542) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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