Pyrin Recombinant Rabbit Monoclonal Antibody [PSH06-12]
Catalog# HA722509
Pyrin Recombinant Rabbit Monoclonal Antibody [PSH06-12]
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WB
-
FC
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Human
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unconjugated
Overview
Product Name
Pyrin Recombinant Rabbit Monoclonal Antibody [PSH06-12]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Pyrin aa 450-781.
Species Reactivity
Human
Validated Applications
WB, FC
Target Molecular Weight
Predicted band size: 86 kDa
Positive Control
THP-1 cell lysate, THP-1 treated with 1μg/mL LPS for 24 hours cell lysate, THP-1.
Conjugation
unconjugated
Clone Number
PSH06-12
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | |
|---|---|---|---|
| human |
|
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
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FC
-
1:1,000
Target
Function
Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence preventing excessive IL1B- and IL18-mediated inflammation. However, it can also have a positive effect in the inflammatory pathway, acting as an innate immune sensor that triggers PYCARD/ASC specks formation, caspase-1 activation, and IL1B and IL18 production. Together with AIM2, also acts as a mediator of pyroptosis, necroptosis and apoptosis (PANoptosis), an integral part of host defense against pathogens, in response to bacterial infection. It is required for PSTPIP1-induced PYCARD/ASC oligomerization and inflammasome formation. Recruits PSTPIP1 to inflammasomes, and is required for PSTPIP1 oligomerization.
Background References
1. Papin S., Cuenin S., Agostini L., Martinon F., Werner S., Beer H.D., Grutter C., Grutter M., Tschopp J. The SPRY domain of Pyrin, mutated in familial Mediterranean fever patients, interacts with inflammasome components and inhibits proIL-1beta processing. Cell Death Differ. 14:1457-1466 (2007)
2. Kimura T., Jain A., Choi S.W., Mandell M.A., Schroder K., Johansen T., Deretic V. TRIM-mediated precision autophagy targets cytoplasmic regulators of innate immunity. J. Cell Biol. 210:973-989 (2015)
Subcellular Location
Cell projection. Cytoplasm. Cytoskeleton. Microtubule. Nucleus.
UNIPROT
Synonyms
FMF antibody
Marenostrin antibody
Mediterranean fever antibody
Mediterranean fever protein antibody
MEF antibody
Mefv antibody
MEFV_HUMAN antibody
Pyrin antibody
TRIM20 antibody
Images
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☑ Cell treatment (CT)
Western blot analysis of Pyrin on different lysates with Rabbit anti-Pyrin antibody (HA722509) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 treated with 1μg/mL LPS for 24 hours cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 86 kDa
Observed band size: 86 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722509) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of THP-1 cells labeling Pyrin.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA722509, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"