PHACTR3 Recombinant Rabbit Monoclonal Antibody [PSH06-09]
Catalog# HA722506
PHACTR3 Recombinant Rabbit Monoclonal Antibody [PSH06-09]
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WB
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IF-Cell
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Human
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unconjugated
Overview
Product Name
PHACTR3 Recombinant Rabbit Monoclonal Antibody [PSH06-09]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human PHACTR3 aa 401-450.
Species Reactivity
Human
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 63 kDa
Positive Control
HepG2 cell lysate, 293T cell lysate, Jurkat cell lysate, HepG2.
Conjugation
unconjugated
Clone Number
PSH06-09
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:50
Target
Function
Phosphatase and actin regulator 3 is an enzyme that in humans is encoded by the PHACTR3 gene. The protein encoded by this gene is associated with the nuclear scaffold in proliferating cells. It was found to bind to the catalytic subunit of protein phosphatase-1 (PP1) and inhibit PP1 activity, suggesting that this protein may function as a regulatory subunit of PP1. Alternative splicing at this locus results in several transcript variants encoding different isoforms.
Background References
1. Miyata T et al. Neuron-enriched phosphatase and actin regulator 3 (Phactr3)/ nuclear scaffold-associated PP1-inhibiting protein (Scapinin) regulates dendritic morphology via its protein phosphatase 1-binding domain. Biochem Biophys Res Commun. 2020 Jul
2. Dragoj M et al. Association of Overexpressed MYC Gene with Altered PHACTR3 and E2F4 Genes Contributes to Non-small Cell Lung Carcinoma Pathogenesis. J Med Biochem. 2019 Mar
Subcellular Location
Nucleus matrix.
UNIPROT
Synonyms
Phosphatase and actin regulator 3
Scaffold-associated PP1-inhibiting protein (Scapinin)
PHACTR3
C20orf101
SCAPIN1
Images
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Western blot analysis of PHACTR3 on different lysates with Rabbit anti-PHACTR3 antibody (HA722506) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: 293T cell lysate
Lane 3: Jurkat cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722506) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HepG2 cells labeling PHACTR3 with Rabbit anti-PHACTR3 antibody (HA722506) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PHACTR3 antibody (HA722506) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"