CD2 Recombinant Rabbit Monoclonal Antibody [PSH05-84]
Catalog# HA722483
CD2 Recombinant Rabbit Monoclonal Antibody [PSH05-84]
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WB
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IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
CD2 Recombinant Rabbit Monoclonal Antibody [PSH05-84]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CD2 aa 25-209 (Extracellular).
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 39 kDa
Positive Control
Jurkat cell lysate, human T cell lymphoma tissue, human appendix tissue, human spleen tissue, human liver tissue.
Conjugation
unconjugated
Clone Number
PSH05-84
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:1,000
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IF-Tissue
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1:500
Target
Function
CD2 is a transmembrane glycoprotein expressed early in thymocyte development and present on most circulating T cells. CD2 plays a role in T cell adhesion through binding to its ligand CD58 (LFA-3). Stimulation of CD2 also leads to T cell activation and proliferation. T cells from mice deficient in both CD2 and CD28 have severe defects in T cell activation and function, while T cells deficient in either CD2 or CD28 are still capable of mounting a response, suggesting that CD2 and CD28 may have overlapping functions and may be able to compensate for each other. In addition, engagement of CD2 and CD58 was recently demonstrated to be the primary costimulatory signal in T cells that lack CD28. CD2 expression also distinguishes a subset of plasmacytoid dendritic cells found in tumors and tonsils that express lysozyme, higher levels of IL-12 p40, and higher levels of CD80.
Background References
1. Judith Leitner, Dietmar Herndler-Brandstetter, Gerhard J Zlabinger, Beatrix Grubeck-Loebenstein, Peter Steinberger. CD58/CD2 Is the Primary Costimulatory Pathway in Human CD28-CD8+ T Cells. J Immunol. 2015 Jul 15;195(2):477-87.
2. Toshimichi Matsui, John E Connolly, Mark Michnevitz, Damien Chaussabel, Chun-I Yu, Casey Glaser, Sasha Tindle, Marc Pypaert, Heidi Freitas, Bernard Piqueras, Jacques Banchereau, A Karolina Palucka. CD2 distinguishes two subsets of human plasmacytoid dendritic cells with distinct phenotype and functions. J Immunol. 2009 Jun 1;182(11):6815-23.
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
CD 2 antibody
CD2 antibody
CD2 antigen (p50), sheep red blood cell receptor antibody
CD2 antigen antibody
CD2 molecule antibody
CD2_HUMAN antibody
Erythrocyte receptor antibody
FLJ46032 antibody
LFA-2 antibody
LFA-3 receptor antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of CD2 on different lysates with Rabbit anti-CD2 antibody (HA722483) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: Raji cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 50 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722483) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human T cell lymphoma tissue with Rabbit anti-CD2 antibody (HA722483) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722483) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-CD2 antibody (HA722483) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722483) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD2 antibody (HA722483) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722483) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CD2 antibody (HA722483) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722483) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: Immunofluorescence (IF-tissue)
Species: Human
Tissue: Tonsil
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA722483, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Tonsil
Sample: Paraffin-embedded section
Primary antibody dilution: 1/1,000
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes
Platform: Leica Biosystems BOND® RX
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CD2 Recombinant Rabbit Monoclonal Antibody [PSH05-85] - BSA and Azide free
Application: WB,IHC-P,IF-Cell,FC,IF-Tissue
Reactivity: Human
Conjugate: unconjugated
