Syndecan 3 Recombinant Rabbit Monoclonal Antibody [JE64-19]
Catalog# HA722471
Syndecan 3 Recombinant Rabbit Monoclonal Antibody [JE64-19]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Syndecan 3 Recombinant Rabbit Monoclonal Antibody [JE64-19]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Syndecan 3 aa 51-100 / 442.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 45 kDa
Positive Control
A549 cell lysate, MCF7 cell lysate, SH-SY5Y cell lysate, Jurkat cell lysate, Mouse lung tissue lysate, Mouse kidney tissue lysate, Rat lung tissue lysate.
Conjugation
unconjugated
Clone Number
JE64-19
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
The protein encoded by this gene belongs to the syndecan proteoglycan family. It may play a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. Allelic variants of this gene have been associated with obesity. Cell surface proteoglycan that may bear heparan sulfate (By similarity). May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.
Background References
1. Berndt C., Casaroli-Marano R.P., Vilaro S., Reina M. Cloning and characterization of human syndecan-3. J. Cell. Biochem. 82:246-259 (2001)
Subcellular Location
Cell membrane.
Synonyms
N-syndecan antibody
Sdc3 antibody
SDC3_HUMAN antibody
SDCN antibody
SYND3 antibody
Syndecan neural type antibody
syndecan proteoglycan 3 antibody
Syndecan-3 antibody
Images
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Western blot analysis of Syndecan 3 on different lysates with Rabbit anti-Syndecan 3 antibody (HA722471) at 1/1,000 dilution.
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: SH-SY5Y cell lysate (20 µg/Lane)
Lane 4: Jurkat cell lysate (20 µg/Lane)
Lane 5: Mouse lung tissue lysate (40 µg/Lane)
Lane 6: Mouse kidney tissue lysate (40 µg/Lane)
Lane 7: Rat lung tissue lysate (40 µg/Lane)
Predicted band size: 45 kDa
Observed band size: 70 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722471) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"