rSec6 Recombinant Rabbit Monoclonal Antibody [JE64-12]
Catalog# HA722470
rSec6 Recombinant Rabbit Monoclonal Antibody [JE64-12]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
rSec6 Recombinant Rabbit Monoclonal Antibody [JE64-12]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human rSec6 aa 671-720 / 745.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 85 kDa
Positive Control
HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, A431 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, C6 cell lysate.
Conjugation
unconjugated
Clone Number
JE64-12
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity. Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
Background References
1. Guo J., Mei K. Structural Study of the Exocyst Subunit Human Sec6. Stud Health Technol Inform 308:351-358 (2023)
2. Tanaka T., Iino M., Goto K. Sec6 enhances cell migration and suppresses apoptosis by elevating the phosphorylation of p38 MAPK, MK2, and HSP27. Cell Signal 49:1-16 (2018)
Subcellular Location
Cell projection. Cytoplasm. Golgi apparatus.
Synonyms
EXOC 3 antibody
Exoc3 antibody
EXOC3_HUMAN antibody
Exocyst complex component 3 antibody
Exocyst complex component Sec 6 antibody
Exocyst complex component Sec6 antibody
rSec 6 antibody
Sec 6 antibody
Sec 6 homolog antibody
Sec 6p antibody
ExpandImages
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Western blot analysis of rSec6 on different lysates with Rabbit anti-rSec6 antibody (HA722470) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: A431 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: Neuro-2a cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722470) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"