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intestinal alkaline phosphatase Recombinant Rabbit Monoclonal Antibody [JE60-27]

Usd: 385

Specification

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Catalog# HA722461

intestinal alkaline phosphatase Recombinant Rabbit Monoclonal Antibody [JE60-27]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

intestinal alkaline phosphatase Recombinant Rabbit Monoclonal Antibody [JE60-27]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human intestinal alkaline phosphatase aa 1-100 / 528.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 57 kDa

Positive Control

Caco-2 cell lysate, SW480 cell lysate, human small intestine tissue, human placenta tissue.

Conjugation

unconjugated

Clone Number

JE60-27

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut. Alkaline phosphatase that can hydrolyze various phosphate compounds. In most mammals there are four different isozymes: placental (ALPP), germ cell (ALPG), intestinal (ALPI) and tissue non-specific (liver/bone/kidney) (ALPL/TNAP).

Background References

1. Malo J., Alam M.J., Shahnaz M., Kaliannan K., Chandra G., Aziz T., Sarker T., Bala M., Paul R., Malo M.S. Intestinal Alkaline Phosphatase Deficiency Is Associated with Ischemic Heart Disease. Dis Markers 2019:8473565-8473565 (2019)

2. Liu W., Hu D., Huo H., Zhang W., Adiliaghdam F., Morrison S., Ramirez J.M., Gul S.S., Hamarneh S.R., Hodin R.A. Intestinal Alkaline Phosphatase Regulates Tight Junction Protein Levels. J Am Coll Surg 222:1009-1017 (2016)

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P09923 Human

Synonyms

Alkaline phosphatase intestinal antibody

Alkaline phosphomonoesterase antibody

Alpi antibody

Glycerophosphatase antibody

IAP antibody

Intestinal alkaline phosphatase antibody

Intestinal-type alkaline phosphatase antibody

Kasahara isozyme antibody

OTTHUMP00000164357 antibody

PPBI_HUMAN antibody

Images

  • Western blot analysis of intestinal alkaline phosphatase on different lysates with Rabbit anti-intestinal alkaline phosphatase antibody (<a href="/products/HA722461" style="font-weight: bold;text-decoration: underline;">HA722461</a>) at 1/1,000 dilution.<br /><br />Lane 1: Caco-2 cell lysate<br />Lane 2: SW480 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 57 kDa<br />Observed band size: 70-75 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722461" style="font-weight: bold;text-decoration: underline;">HA722461</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of intestinal alkaline phosphatase on different lysates with Rabbit anti-intestinal alkaline phosphatase antibody (HA722461) at 1/1,000 dilution.

    Lane 1: Caco-2 cell lysate
    Lane 2: SW480 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 57 kDa
    Observed band size: 70-75 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722461) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-intestinal alkaline phosphatase antibody (<a href="/products/HA722461" style="font-weight: bold;text-decoration: underline;">HA722461</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722461" style="font-weight: bold;text-decoration: underline;">HA722461</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-intestinal alkaline phosphatase antibody (HA722461) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722461) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-intestinal alkaline phosphatase antibody (<a href="/products/HA722461" style="font-weight: bold;text-decoration: underline;">HA722461</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722461" style="font-weight: bold;text-decoration: underline;">HA722461</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-intestinal alkaline phosphatase antibody (HA722461) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722461) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"