ABO Recombinant Rabbit Monoclonal Antibody [JE39-25]
Catalog# HA722455
ABO Recombinant Rabbit Monoclonal Antibody [JE39-25]
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WB
-
IP
-
Human
-
Rat
-
unconjugated
Overview
Product Name
ABO Recombinant Rabbit Monoclonal Antibody [JE39-25]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human ABO aa 1-50 / 354.
Species Reactivity
Human, Rat
Validated Applications
WB, IP
Target Molecular Weight
Predicted band size: 41 kDa
Positive Control
HT-29 cell lysate, K-562 cell lysate, 293T cell lysate, rat skeletal muscle tissue lysate.
Conjugation
unconjugated
Clone Number
JE39-25
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IP
-
1-2μg/sample
Target
Function
This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.
Background References
1. Letts J.A., Rose N.L., Fang Y.R., Barry C.H., Borisova S.N., Seto N.O.L., Palcic M.M., Evans S.V. Differential recognition of the type I and II H antigen acceptors by the human ABO(H) blood group A and B glycosyltransferases. J. Biol. Chem. 281:3625-3632 (2006)
2. Posch-Pertl L., List W., Michelitsch M., Pinter-Hausberger S., Posch F., Innauer F., Renner W., Weger M. Role of the ABO Blood Groups as a Risk Factor for Retinal Vein Occlusion. Ophthalmic Res 66:164-169 (2023)
Subcellular Location
Golgi apparatus, Golgi stack membrane. Secreted.
UNIPROT
Synonyms
A transferase antibody
Abo antibody
B transferase antibody
BGAT_HUMAN antibody
Blood Group H2 antigen antibody
CD173 antibody
Fucosylglycoprotein 3-alpha-galactosyltransferase antibody
Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase antibody
Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form antibody
Glycoprotein-fucosylgalactoside alpha-galactosyltransferase antibody
ExpandImages
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Western blot analysis of ABO on different lysates with Rabbit anti-ABO antibody (HA722455) at 1/1,000 dilution.
Lane 1: HT-29 cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: Rat skeletal muscle tissue lysate (40 µg/Lane)
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722455) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
ABO was immunoprecipitated from 0.2 mg 293T cell lysate with HA722455 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722455 at 1/1,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate (input)
Lane 2: HA722455 IP in 293T cell lysate
Lane 3: Rabbit IgG instead of HA722455 in 293T cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute 2 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"