Smg1 Recombinant Rabbit Monoclonal Antibody [JE38-38]
Catalog# HA722452
Smg1 Recombinant Rabbit Monoclonal Antibody [JE38-38]
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WB
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Human
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Mouse
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Rat
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Green monkey
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unconjugated
Overview
Product Name
Smg1 Recombinant Rabbit Monoclonal Antibody [JE38-38]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Smg1 aa 3,562-3,661 / 3,661.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB
Target Molecular Weight
Predicted band size: 410 kDa
Positive Control
HEK-293 cell lysate, MCF7 cell lysate, C2C12 cell lysate, C6 cell lysate, COS-1 cell lysate.
Conjugation
unconjugated
Clone Number
JE38-38
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ.
Background References
1. Denning G., Jamieson L., Maquat L.E., Thompson E.A., Fields A.P. Cloning of a novel phosphatidylinositol kinase-related kinase: characterization of the human SMG-1 RNA surveillance protein. J. Biol. Chem. 276:22709-22714 (2001)
2. Yamashita A., Ohnishi T., Kashima I., Taya Y., Ohno S. Human SMG-1, a novel phosphatidylinositol 3-kinase-related protein kinase, associates with components of the mRNA surveillance complex and is involved in the regulation of nonsense-mediated mRNA decay. Genes Dev. 15:2215-2228 (2001)
Subcellular Location
Cytoplasm. Nucleus.
Synonyms
61E3.4 antibody
ATX antibody
hSMG-1 antibody
hSMG1 antibody
KIAA0421 antibody
Lambda interacting protein antibody
Lambda-interacting protein antibody
Lambda/iota protein kinase C interacting protein antibody
Lambda/iota protein kinase C-interacting protein antibody
LIP antibody
ExpandImages
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Western blot analysis of Smg1 on different lysates with Rabbit anti-Smg1 antibody (HA722452) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: C2C12 cell lysate
Lane 4: C6 cell lysate
Lane 5: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 410 kDa
Observed band size: 410 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
3-8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722452) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"