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p40/RABEPK Recombinant Rabbit Monoclonal Antibody [JE35-06]

Usd: 385

Specification

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Catalog# HA722449

p40/RABEPK Recombinant Rabbit Monoclonal Antibody [JE35-06]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

p40/RABEPK Recombinant Rabbit Monoclonal Antibody [JE35-06]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human p40/RABEPK aa 223-372 / 372.

Species Reactivity

Human

Validated Applications

WB

Target Molecular Weight

Predicted band size: 40 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, U-87 MG cell lysate, A549 cell lysate.

Conjugation

unconjugated

Clone Number

JE35-06

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Predicted to be involved in receptor-mediated endocytosis and vesicle docking involved in exocytosis. Predicted to be located in cytoplasmic vesicle; cytosol; and trans-Golgi network membrane.

Background References

1. Diaz E., Schimmoller F., Pfeffer S.R. A novel Rab9 effector required for endosome-to-TGN transport. J. Cell Biol. 138:283-290 (1997)

Subcellular Location

Cytoplasm. Endosome membrane.

UNIPROT

SwissProt: Q7Z6M1 Human

Synonyms

40 kDa Rab9 effector protein antibody

8430412M01Rik antibody

9530020d24rik antibody

AV073337 antibody

bA65N13.1 antibody

C87311 antibody

DKFZp686P1077 antibody

OTTHUMP00000022126 antibody

OTTMUSP00000012898 antibody

OTTMUSP00000012899 antibody

Expand

Images

  • Western blot analysis of p40/RABEPK on different lysates with Rabbit anti-p40/RABEPK antibody (<a href="/products/HA722449" style="font-weight: bold;text-decoration: underline;">HA722449</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: U-87 MG cell lysate<br />Lane 4: A549 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 40 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722449" style="font-weight: bold;text-decoration: underline;">HA722449</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of p40/RABEPK on different lysates with Rabbit anti-p40/RABEPK antibody (HA722449) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: U-87 MG cell lysate
    Lane 4: A549 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722449) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"