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IL-1RA Recombinant Rabbit Monoclonal Antibody [JE07-58]

Usd: 385

Specification

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Catalog# HA722446

IL-1RA Recombinant Rabbit Monoclonal Antibody [JE07-58]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

IL-1RA Recombinant Rabbit Monoclonal Antibody [JE07-58]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human IL-1RA aa 78-177 / 177.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 20 kDa

Positive Control

A431 cell lysate, SW480 cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, Mouse lung tissue lysate, C6 cell lysate, Rat lung tissue lysate, Rat brain tissue lysate, Rat kidney tissue lysate, Rat spleen tissue lysate.

Conjugation

unconjugated

Clone Number

JE07-58

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens. The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses, particularly in the acute phase of infection and inflammation. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.

Background References

1. Greenfeder S.A., Nunes P., Kwee L., Labow M., Chizzonite R.A., Ju G. Molecular cloning and characterization of a second subunit of the interleukin 1 receptor complex. J. Biol. Chem. 270:13757-13765 (1995)

Subcellular Location

Cytoplasm. Secreted.

UNIPROT

SwissProt: P18510 Human

SwissProt: P25085 Mouse

SwissProt: P25086 Rat

Synonyms

DIRA antibody

F630041P17Rik antibody

ICIL 1RA antibody

ICIL-1RA antibody

ICIL1RA antibody

IL-1ra antibody

IL-1ra3 antibody

IL-1RN antibody

IL1 inhibitor antibody

IL1F3 antibody

Expand

Images

  • Western blot analysis of IL-1RA on different lysates with Rabbit anti-IL-1RA antibody (<a href="/products/HA722446" style="font-weight: bold;text-decoration: underline;">HA722446</a>) at 1/1,000 dilution.<br /><br />Lane 1: A431 cell lysate (20 µg/Lane)<br />Lane 2: SW480 cell lysate (20 µg/Lane)<br />Lane 3: Neuro-2a cell lysate (20 µg/Lane)<br />Lane 4: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 5: C2C12 cell lysate (20 µg/Lane)<br />Lane 6: Mouse lung tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 20 kDa<br />Observed band size: 20 kDa<br /><br />Exposure time: 4 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722446" style="font-weight: bold;text-decoration: underline;">HA722446</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of IL-1RA on different lysates with Rabbit anti-IL-1RA antibody (HA722446) at 1/1,000 dilution.

    Lane 1: A431 cell lysate (20 µg/Lane)
    Lane 2: SW480 cell lysate (20 µg/Lane)
    Lane 3: Neuro-2a cell lysate (20 µg/Lane)
    Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 5: C2C12 cell lysate (20 µg/Lane)
    Lane 6: Mouse lung tissue lysate (40 µg/Lane)

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

    Exposure time: 4 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722446) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of IL-1RA on different lysates with Rabbit anti-IL-1RA antibody (<a href="/products/HA722446" style="font-weight: bold;text-decoration: underline;">HA722446</a>) at 1/1,000 dilution.<br /><br />Lane 1: C2C12 cell lysate (20 µg/Lane)<br />Lane 2: C6 cell lysate (20 µg/Lane)<br />Lane 3: Rat lung tissue lysate (40 µg/Lane)<br />Lane 4: Rat brain tissue lysate (40 µg/Lane)<br />Lane 5: Rat kidney tissue lysate (40 µg/Lane)<br />Lane 6: Rat spleen tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 20 kDa<br />Observed band size: 20 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722446" style="font-weight: bold;text-decoration: underline;">HA722446</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of IL-1RA on different lysates with Rabbit anti-IL-1RA antibody (HA722446) at 1/1,000 dilution.

    Lane 1: C2C12 cell lysate (20 µg/Lane)
    Lane 2: C6 cell lysate (20 µg/Lane)
    Lane 3: Rat lung tissue lysate (40 µg/Lane)
    Lane 4: Rat brain tissue lysate (40 µg/Lane)
    Lane 5: Rat kidney tissue lysate (40 µg/Lane)
    Lane 6: Rat spleen tissue lysate (40 µg/Lane)

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722446) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"