APPL Recombinant Rabbit Monoclonal Antibody [JE35-02] (HA722430)

UNIPROT #

Q9UKG1

PROTEIN NAME

APPL

SYNONYMS

Adapter protein containing PH domain antibody;Adaptor Protein Containing pH Domain antibody;Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody;Adaptor protein containing pH domain PTB domain and leucine zipper motif antibody;Adaptor protein phosphotyrosine interacting with PH domain and leucine zipper 1 antibody;AKT2 INTERACTOR antibody;APPL 1 antibody;Appl1 antibody;DCC interacting protein 13 alpha antibody;DCC-interacting protein 13-alpha antibody;DIP 13 alpha antibody;DIP13 alpha antibody;Dip13-alpha antibody;DIP13alpha antibody;DP13A_HUMAN antibody;KIAA1428 antibody;PTB domain and leucine zipper motif 1 antibody;PTB Domain and Leucine Zipper Motif antibody;Signaling adaptor protein DIP13alpha antibody

SUBCELLULAR LOCATION

Early endosome membrane, Nucleus, Cytoplasm, Endosome, Cell projection, ruffle, Cytoplasmic vesicle, phagosome.

FUNCTION

Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), or DCC-interacting protein 13-alpha (DIP13alpha), is a protein that in humans is encoded by the APPL1 gene. APPL1 contains several key interactory domains: pleckstrin homology (PH) domain, phosphotyrosine-binding (PTB) domain and Bin–Amphiphysin–Rvs (BAR) domain. APPL1 is an adaptor protein localized to a subset of Rab5-positive ("early") endosomes, where it recruits other binding partners and regulates vesicle trafficking and endosomal signalling. APPL1 is enriched at very early endosomes which are negative for EEA1, indicating that APPL1 affects the earliest stages of endosomal traffic before EEA1 takes over. This is in line with observations that APPL1 and EEA1 compete for Rab5 binding. APPL1 affects the speed of internalization of key endosomal cargo (eg. EGF receptor) which is dependent on Rab5 activation. PTB domain of APPL1 regulates many cell signalling events in specific endosomal compartments - sometimes termed the "signalling endosomes". This includes lysophosphatidic acid (LPA)-induced signaling (together with interacting protein GIPC1). Additional roles for APPL1 were pinpointed to the nucleus where APPL1 can localize once dissociated from endosomes.