MRC2 Recombinant Rabbit Monoclonal Antibody [JE34-76] (HA722429)
Catalog# HA722429
Rabbit Monoclonal to MRC2
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of MRC2 on different lysates with Rabbit anti-MRC2 antibody (HA722429) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: HEK-293 cell lysate treated with deglycosylation
Lane 3: Saos-2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 167 kDa
Observed band size: 167-250 kDa
Exposure time: Lane 1-2: 3 minutes; Lane 3: 4 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722429) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of MRC2 on different lysates with Rabbit anti-MRC2 antibody (HA722429) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: HEK-293 cell lysate treated with deglycosylation
Lane 3: Saos-2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 167 kDa
Observed band size: 167-250 kDa
Exposure time: Lane 1-2: 3 minutes; Lane 3: 4 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722429) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.