ADAM15 Recombinant Rabbit Monoclonal Antibody [JE39-01]
Catalog# HA722402
ADAM15 Recombinant Rabbit Monoclonal Antibody [JE39-01]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
ADAM15 Recombinant Rabbit Monoclonal Antibody [JE39-01]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human ADAM15 aa 250-350 (extracellular).
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 93 kDa
Positive Control
SW480 cell lysate, HUVEC cell lysate, A431 cell lysate, NIH/3T3 cell lysate, mouse colon tissue lysate, rat colon tissue lysate, human colon tissue, human liver tissue, human small intestine tissue.
Conjugation
unconjugated
Clone Number
JE39-01
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
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IHC-P
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1:1,000
Target
Function
Disintegrin and metalloproteinase domain-containing protein 15 is an enzyme that in humans is encoded by the ADAM15 gene. The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as an EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.
Background References
1. Chute M et al. ADAM15 is required for optimal collagen cross-linking and scar formation following myocardial infarction. Matrix Biol. 2022 Jan
2. Puig-Blasco L et al. Loss of cancer cell-derived ADAM15 alters the tumor microenvironment in colorectal tumors. Int J Cancer. 2023 Dec
Subcellular Location
Endomembrane system, Cell junction, adherens junction, Cell projection, cilium, flagellum, Cytoplasmic vesicle, secretory vesicle, acrosome.
Synonyms
A disintegrin and metalloproteinase domain 15 (metargidin) antibody
A disintegrin and metalloproteinase domain 15 antibody
ADA15_HUMAN antibody
ADAM 15 antibody
ADAM metallopeptidase domain 15 antibody
Adam15 antibody
and cysteine-rich protein 15 antibody
Disintegrin and metalloproteinase domain-containing protein 15 antibody
disintegrin-like antibody
EC 3.4.24. antibody
ExpandImages
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Western blot analysis of ADAM15 on different lysates with Rabbit anti-ADAM15 antibody (HA722402) at 1/1,000 dilution.
Lane 1: SW480 cell lysate (15 µg/Lane)
Lane 2: HUVEC cell lysate (15 µg/Lane)
Lane 3: A431 cell lysate (15 µg/Lane)
Lane 4: NIH/3T3 cell lysate (15 µg/Lane)
Lane 5: Mouse colon tissue lysate (20 µg/Lane)
Lane 6: Rat colon tissue lysate (20 µg/Lane)
Predicted band size: 93 kDa
Observed band size: 85 kDa
Exposure time: 25 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722402) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ADAM15 antibody (HA722402) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722402) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ADAM15 antibody (HA722402) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722402) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-ADAM15 antibody (HA722402) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722402) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"