KLC3 Recombinant Rabbit Monoclonal Antibody [JE77-61]
Catalog# HA722375
KLC3 Recombinant Rabbit Monoclonal Antibody [JE77-61]
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WB
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IF-Cell
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
KLC3 Recombinant Rabbit Monoclonal Antibody [JE77-61]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human KLC3 aa 350-450.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size: 55 kDa
Positive Control
Jurkat cell lysate, U-87 MG cell lysate, HepG2 cell lysate, BxPC-3 cell lysate, human liver tissue lysate, mouse testis tissue lysate, HepG2.
Conjugation
unconjugated
Clone Number
JE77-61
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | IP | |
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| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:500
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FC
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1:1,000
Target
Function
Kinesin light chain 3 is a protein that in humans is encoded by the KLC3 gene. This gene encodes a member of the kinesin light chain gene family. Kinesins are molecular motors involved in the transport of cargo along microtubules, and are composed of two kinesin heavy chain (KHC) and two kinesin light chain (KLC) molecules. KLCs are thought to typically be involved in binding cargo and regulating kinesin activity. In the rat, a protein similar to this gene product is expressed in post-meiotic spermatids, where it associates with structural components of sperm tails and mitochondria.
Background References
1. Rah G et al. KLC3 Regulates Ciliary Trafficking and Cyst Progression in CILK1 Deficiency-Related Polycystic Kidney Disease. J Am Soc Nephrol. 2022 Sep
Subcellular Location
Cytoplasm, cytoskeleton, Mitochondrion.
Synonyms
Kinesin light chain 2 antibody
Kinesin light chain 3 antibody
KLC2 antibody
KLC2 like antibody
KLC2-like antibody
KLC2L antibody
KLC3 antibody
KLC3_HUMAN antibody
KLCt antibody
KNS2B antibody
Images
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Western blot analysis of KLC3 on different lysates with Rabbit anti-KLC3 antibody (HA722375) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: U-87 MG cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: BxPC-3 cell lysate (20 µg/Lane)
Lane 5: Human liver tissue lysate (40 µg/Lane)
Lane 6: Mouse testis tissue lysate (40 µg/Lane)
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722375) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HepG2 cells labeling KLC3 with Rabbit anti-KLC3 antibody (HA722375) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KLC3 antibody (HA722375) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of HepG2 cells labeling KLC3.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA722375, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"