Meckelin Recombinant Rabbit Monoclonal Antibody [JE65-62]
Catalog# HA722366
Meckelin Recombinant Rabbit Monoclonal Antibody [JE65-62]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Meckelin Recombinant Rabbit Monoclonal Antibody [JE65-62]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Meckelin aa 801-900 / 995.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 112 kDa
Positive Control
MCF7 cell lysate, 293T cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, mouse spleen tissue lysate, mouse brain tissue lysate, rat spleen tissue lysate, rat brain tissue lysate, mouse liver tissue, mouse kidney tissue, rat kidney tissue.
Conjugation
unconjugated
Clone Number
JE65-62
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
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IHC-P
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1:1,000
Target
Function
Meckelin is a protein that in humans is encoded by the TMEM67 gene. The protein encoded by this gene localizes to the primary cilium and to the plasma membrane. The gene functions in centriole migration to the apical membrane and formation of the primary cilium. Multiple transcript variants encoding different isoforms have been found for this gene.
Background References
1. Yinsheng Z et al. TMEM67 is required for the gating function of the transition zone that controls entry of membrane-associated proteins ARL13B and INPP5E into primary cilia. Biochem Biophys Res Commun. 2022 Dec
2. Qiu YL et al. Association of novel TMEM67 variants with mild phenotypes of high gamma-glutamyl transpeptidase cholestasis and congenital hepatic fibrosis. J Cell Physiol. 2022 Jun
Subcellular Location
Cell membrane, Endoplasmic reticulum membrane, Cell projection, cilium, Cytoplasm, cytoskeleton, cilium basal body.
Synonyms
JBTS6 antibody
Meckel syndrome type 3 protein antibody
MKS3 antibody
NPHP11 antibody
TMEM67 antibody
TNEM67 antibody
Transmembrane protein 67 antibody
Images
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Western blot analysis of Meckelin on different lysates with Rabbit anti-Meckelin antibody (HA722366) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: 293T cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 112 kDa
Observed band size: 112 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722366) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Meckelin on different lysates with Rabbit anti-Meckelin antibody (HA722366) at 1/1,000 dilution.
Lane 1: Mouse spleen tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat spleen tissue lysate
Lane 4: Rat brain tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 112 kDa
Observed band size: 112 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722366) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Meckelin antibody (HA722366) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722366) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Meckelin antibody (HA722366) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722366) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Meckelin antibody (HA722366) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722366) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"