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TAPP-1 Recombinant Rabbit Monoclonal Antibody [JE64-38]

Usd: 385

Specification

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Catalog# HA722361

TAPP-1 Recombinant Rabbit Monoclonal Antibody [JE64-38]

Application

  • WB

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

Conjugation

  • unconjugated

Overview

Product Name

TAPP-1 Recombinant Rabbit Monoclonal Antibody [JE64-38]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human TAPP-1 aa 300-400.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 45 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, Neuro-2a cell lysate, C6 cell lysate, COS-1 cell lysate, HeLa.

Conjugation

unconjugated

Clone Number

JE64-38

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:1,000

Target

Function

This gene encodes a pleckstrin homology domain-containing adapter protein. The encoded protein is localized to the plasma membrane where it specifically binds phosphatidylinositol 3,4-bisphosphate. This protein may be involved in the formation of signaling complexes in the plasma membrane. Polymorphisms in this gene are associated with age-related macular degeneration. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 5.

Background References

1. Mei R et al. TAPP1 Represses the Differentiation of Oligodendrocyte and its Deficiency Accelerates Myelin Regeneration after Demyelinating Injuries. Neurosci Bull. 2021 Mar

2. Chen Y et al. TAPP1 inhibits the differentiation of oligodendrocyte precursor cells via suppressing the Mek/Erk pathway. Neurosci Bull. 2015 Oct

Subcellular Location

Cytoplasm, Cell membrane, Nucleus.

UNIPROT

SwissProt: Q9HB21 Human

SwissProt: Q8BUL6 Mouse

Entrez Gene: 361659 Rat

Synonyms

OTTHUMP00000020651 antibody

PH domain containing family A member 1 antibody

PH domain-containing family A member 1 antibody

PKHA1_HUMAN antibody

Pleckstrin homology domain containing A1 antibody

Pleckstrin homology domain containing family A member 1 antibody

Pleckstrin homology domain containing, family A (phosphoinositide binding specific) member antibody

Pleckstrin homology domain-containing family A member 1 antibody

PLEKHA1 antibody

Tandem PH domain containing protein 1 antibody

Expand

Images

  • Western blot analysis of TAPP-1 on different lysates with Rabbit anti-TAPP-1 antibody (<a href="/products/HA722361" style="font-weight: bold;text-decoration: underline;">HA722361</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: Neuro-2a cell lysate<br />Lane 4: C6 cell lysate<br />Lane 5: COS-1 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 45 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722361" style="font-weight: bold;text-decoration: underline;">HA722361</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TAPP-1 on different lysates with Rabbit anti-TAPP-1 antibody (HA722361) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: Neuro-2a cell lysate
    Lane 4: C6 cell lysate
    Lane 5: COS-1 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722361) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of HeLa cells labeling TAPP-1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA722361" style="font-weight: bold;text-decoration: underline;">HA722361</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HeLa cells labeling TAPP-1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA722361, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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