C3orf38 Recombinant Rabbit Monoclonal Antibody [JE64-28]
Catalog# HA722360
C3orf38 Recombinant Rabbit Monoclonal Antibody [JE64-28]
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WB
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IF-Cell
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Human
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Green monkey
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unconjugated
Overview
Product Name
C3orf38 Recombinant Rabbit Monoclonal Antibody [JE64-28]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human C3orf38 aa 1-200 / 329.
Species Reactivity
Human, Green monkey
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 38 kDa
Positive Control
U-2 OS cell lysate, U-87 MG cell lysate, COS-1 cell lysate, U-2 OS.
Conjugation
unconjugated
Clone Number
JE64-28
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | |
|---|---|---|---|---|
| human |
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| mouse |
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| rat |
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| green monkey |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:50
Target
Function
Chromosome 3 open reading frame 38 (C3orf38) is a protein which in humans is encoded by the C3orf38 gene. The C3orf38 protein is 329 amino acids in length. A large domain of unknown function, DUF4518, encompasses majority of the C3orf38 protein. This domain is a part of the protein family pfam15008, which is thought to be involved in apoptosis regulation. This pfam15008 is the only member of the cl20886 superfamily. While the C3orf38 protein does not have any abnormal amino acid abundance as a whole, the DUF4518 has a high abundance of histidines and a low abundance of serines, according to compositional analysis.
Background References
暂无
Subcellular Location
Nucleus.
UNIPROT
Synonyms
C3orf38 antibody
CC038_HUMAN antibody
Chromosome 3 open reading frame 38 antibody
FLJ54270 antibody
Hypothetical protein LOC285237 antibody
MGC26717 antibody
Uncharacterized protein C3orf38 antibody
Images
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Western blot analysis of C3orf38 on different lysates with Rabbit anti-C3orf38 antibody (HA722360) at 1/1,000 dilution.
Lane 1: U-2 OS cell lysate
Lane 2: U-87 MG cell lysate
Lane 3: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722360) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of U-2 OS cells labeling C3orf38 with Rabbit anti-C3orf38 antibody (HA722360) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-C3orf38 antibody (HA722360) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"