ZNF217 Recombinant Rabbit Monoclonal Antibody [JE39-40]
Catalog# HA722344
ZNF217 Recombinant Rabbit Monoclonal Antibody [JE39-40]
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WB
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Human
-
unconjugated
Overview
Product Name
ZNF217 Recombinant Rabbit Monoclonal Antibody [JE39-40]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human ZNF217 aa 1000-1048.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 115 kDa
Positive Control
MCF7 cell lysate, PANC-1 cell lysate, HEK-293 cell lysate.
Conjugation
unconjugated
Clone Number
JE39-40
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Zinc finger protein 217, also known as ZNF217, is a protein which in humans is encoded by the ZNF217 gene. ZNF217 can attenuate apoptotic signals resulting from telomere dysfunction and may promote neoplastic transformation and later stages of malignancy. Znf217 was shown to be a prognostic biomarker and therapeutic target during breast cancer progression.
Background References
1. Fahmé P et al. The Intricate Interplay between the ZNF217 Oncogene and Epigenetic Processes Shapes Tumor Progression. Cancers (Basel). 2022 Dec
2. Li Y et al. ZNF217: the cerberus who fails to guard the gateway to lethal malignancy. Am J Cancer Res. 2021 Jul
Subcellular Location
Nucleus.
UNIPROT
Synonyms
ZABC 1 antibody
ZABC1 antibody
Zinc finger protein 217 antibody
ZN 217 antibody
ZN217 antibody
ZN217_HUMAN antibody
ZNF 217 antibody
ZNF217 antibody
Images
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Western blot analysis of ZNF217 on different lysates with Rabbit anti-ZNF217 antibody (HA722344) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: PANC-1 cell lysate
Lane 3: HEK-293 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 115 kDa
Observed band size: 140 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722344) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"