SRD5A2 Recombinant Rabbit Monoclonal Antibody [JE39-31]
Catalog# HA722343
SRD5A2 Recombinant Rabbit Monoclonal Antibody [JE39-31]
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WB
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Human
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Rat
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unconjugated
Overview
Product Name
SRD5A2 Recombinant Rabbit Monoclonal Antibody [JE39-31]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human SRD5A2 aa 1-100 / 254.
Species Reactivity
Human, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 28 kDa
Positive Control
LNCaP cell lysate, MCF7cell lysate, rat testis tissue lysate, SH-SY5Y cell lysate, HepG2 cell lysate, rat liver tissue lysate.
Conjugation
unconjugated
Clone Number
JE39-31
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
The human gene SRD5A2 encodes the 3-oxo-5α-steroid 4-dehydrogenase 2 enzyme, also known as 5α-reductase type 2 (5αR2), one of three isozymes of 5α-reductase. 5αR2 catalyzes the conversion of the male sex hormone testosterone into the more potent androgen, dihydrotestosterone. 5αR2 is a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The enzyme is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in 5αR2 activity of the can lead to a condition known as 5α-reductase 2 deficiency, which is a cause of 46,XY DSD that presents as atypical male genitalia.
Background References
1. Seo J et al. The Genotype-Phenotype Correlation in Human 5alpha-Reductase Type 2 Deficiency: Classified and Analyzed from a SRD5A2 Structural Perspective. Int J Mol Sci. 2023 Feb
2. Duan H et al. Two genetic variants in the SRD5A2 gene are found to be associated with sex differences in the disease characteristics of patients with chronic hepatitis B virus infection. Biol Sex Differ. 2023 Oct
Subcellular Location
Microsome membrane, Endoplasmic reticulum membrane.
Synonyms
3 oxo 5 alpha steroid 4 dehydrogenase 2 antibody
3-oxo-5-alpha-steroid 4-dehydrogenase 2 antibody
5 alpha SR2 antibody
5 alpha-SR2 antibody
5ART2 antibody
e II 5 alpha reductase antibody
EC antibody
MGC138457 antibody
Micropenis, included antibody
S5A2_HUMAN antibody
ExpandImages
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Western blot analysis of SRD5A2 on different lysates with Rabbit anti-SRD5A2 antibody (HA722343) at 1/1,000 dilution.
Lane 1: LNCaP cell lysate (20 µg/Lane)
Lane 2: MCF7cell lysate (20 µg/Lane)
Lane 3: Rat testis tissue lysate (40 µg/Lane)
Lane 4: SH-SY5Y cell lysate (20 µg/Lane)
Lane 5: HepG2 cell lysate (20 µg/Lane)
Lane 6: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: Lane 1-3: 3 minutes; Lane 4-5: 1 minute; Lane 6: 9 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722343) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"