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EHD1 Recombinant Rabbit Monoclonal Antibody [JE38-59]

Usd: 385

Specification

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Catalog# HA722340

EHD1 Recombinant Rabbit Monoclonal Antibody [JE38-59]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

EHD1 Recombinant Rabbit Monoclonal Antibody [JE38-59]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human EHD1 aa 1-50 / 534.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IP

Target Molecular Weight

Predicted band size: 61 kDa

Positive Control

HeLa cell lysate, SK-OV-3 cell lysate, mouse lung tissue lysate, mouse brain tissue lysate, rat lung tissue lysate, rat brain tissue lysate, human thyroid carcinoma tissue, human lung tissue, mouse lung tissue, rat lung tissue.

Conjugation

unconjugated

Clone Number

JE38-59

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:1,000

  • IP

  • 1-2μg/sample

Target

Function

EH domain-containing protein 1, also known as testilin or PAST homolog 1 (PAST1), is a protein that in humans is encoded by the EHD1 gene belonging to the EHD protein family. This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors.

Background References

1. Xie S et al. EHD1 promotes CP110 ubiquitination by centriolar satellite delivery of HERC2 to the mother centriole. EMBO Rep. 2023 Jun

2. Meindl K et al. A missense mutation in Ehd1 associated with defective spermatogenesis and male infertility. Front Cell Dev Biol. 2023 Oct

Subcellular Location

Recycling endosome membrane, Early endosome membrane, Cell membrane, Cell projection, cilium membrane.

UNIPROT

SwissProt: Q9H4M9 Human

SwissProt: Q9WVK4 Mouse

SwissProt: Q641Z6 Rat

Synonyms

CDABP0131 antibody

EH domain containing 1 antibody

EH domain-containing protein 1 antibody

EHD1 antibody

EHD1_HUMAN antibody

FLJ42622 antibody

FLJ44618 antibody

H PAST antibody

hPAST1 antibody

PAST antibody

Expand

Images

  • Western blot analysis of EHD1 on different lysates with Rabbit anti-EHD1 antibody (<a href="/products/HA722340" style="font-weight: bold;text-decoration: underline;">HA722340</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: SK-OV-3 cell lysate<br />Lane 3: Mouse lung tissue lysate<br />Lane 4: Mouse brain tissue lysate<br />Lane 5: Rat lung tissue lysate<br />Lane 6: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 61 kDa<br />Observed band size: 61 kDa<br /><br />Exposure time: 12 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722340" style="font-weight: bold;text-decoration: underline;">HA722340</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of EHD1 on different lysates with Rabbit anti-EHD1 antibody (HA722340) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: SK-OV-3 cell lysate
    Lane 3: Mouse lung tissue lysate
    Lane 4: Mouse brain tissue lysate
    Lane 5: Rat lung tissue lysate
    Lane 6: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 61 kDa
    Observed band size: 61 kDa

    Exposure time: 12 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722340) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-EHD1 antibody (<a href="/products/HA722340" style="font-weight: bold;text-decoration: underline;">HA722340</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722340" style="font-weight: bold;text-decoration: underline;">HA722340</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-EHD1 antibody (HA722340) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722340) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-EHD1 antibody (<a href="/products/HA722340" style="font-weight: bold;text-decoration: underline;">HA722340</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722340" style="font-weight: bold;text-decoration: underline;">HA722340</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-EHD1 antibody (HA722340) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722340) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-EHD1 antibody (<a href="/products/HA722340" style="font-weight: bold;text-decoration: underline;">HA722340</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722340" style="font-weight: bold;text-decoration: underline;">HA722340</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-EHD1 antibody (HA722340) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722340) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-EHD1 antibody (<a href="/products/HA722340" style="font-weight: bold;text-decoration: underline;">HA722340</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722340" style="font-weight: bold;text-decoration: underline;">HA722340</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-EHD1 antibody (HA722340) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722340) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • EHD1 was immunoprecipitated from 0.2 mg HeLa cell lysate with <a href="/products/HA722340" style="font-weight: bold;text-decoration: underline;">HA722340</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722340" style="font-weight: bold;text-decoration: underline;">HA722340</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs&reg; VHH(HRP) (<a href="/products/025-100-005" style="font-weight: bold;text-decoration: underline;">025-100-005</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: <a href="/products/HA722340" style="font-weight: bold;text-decoration: underline;">HA722340</a> IP in HeLa cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722340" style="font-weight: bold;text-decoration: underline;">HA722340</a> in HeLa cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 5 seconds; ECL: <a href="/products/K1802" style="font-weight: bold;text-decoration: underline;">K1802</a>

    EHD1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722340 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722340 at 1/1,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: HA722340 IP in HeLa cell lysate
    Lane 3: Rabbit IgG instead of HA722340 in HeLa cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 5 seconds; ECL: K1802

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