Skip to content

LHX3 Recombinant Rabbit Monoclonal Antibody [JE37-44]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA722334

LHX3 Recombinant Rabbit Monoclonal Antibody [JE37-44]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

LHX3 Recombinant Rabbit Monoclonal Antibody [JE37-44]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human LHX3/LIM aa 300-397.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 43 kDa

Positive Control

HeLa cell lysate, Jurkat cell lysate, Neuro-2a cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

JE37-44

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

LIM/homeobox protein Lhx3 is a protein that in humans is encoded by the LHX3 gene. LHX3 encodes a protein of a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor that is required for pituitary development and motor neuron specification. Two transcript variants encoding distinct isoforms have been identified for this gene. Mutations in this gene have been associated with a syndrome of combined pituitary hormone deficiency and rigid cervical spine.

Background References

1. Thaiwong T et al. Dwarfism in Tibetan Terrier dogs with an LHX3 mutation. J Vet Diagn Invest. 2021 Jul

2. Pickett CJ et al. Lhx3/4 initiates a cardiopharyngeal-specific transcriptional program in response to widespread FGF signaling. PLoS Biol. 2024 Jan

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q9UBR4 Human

SwissProt: P50481 Mouse

Entrez Gene: 170671 Rat

Synonyms

CPHD 3 antibody

CPHD3 antibody

DKFZp762A2013 antibody

LHX 3 antibody

LHX3 antibody

LHX3_HUMAN antibody

LIM 3 antibody

LIM homeobox 3 antibody

LIM homeobox gene 3 antibody

LIM homeobox protein 3 antibody

Expand

Images

  • Western blot analysis of LHX3 on different lysates with Rabbit anti-LHX3 antibody (<a href="/products/HA722334" style="font-weight: bold;text-decoration: underline;">HA722334</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: Jurkat cell lysate (20 µg/Lane)<br />Lane 3: Neuro-2a cell lysate (20 µg/Lane)<br />Lane 4: C6 cell lysate (20 µg/Lane)<br />Lane 5: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 6: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 43 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722334" style="font-weight: bold;text-decoration: underline;">HA722334</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of LHX3 on different lysates with Rabbit anti-LHX3 antibody (HA722334) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: Jurkat cell lysate (20 µg/Lane)
    Lane 3: Neuro-2a cell lysate (20 µg/Lane)
    Lane 4: C6 cell lysate (20 µg/Lane)
    Lane 5: Mouse brain tissue lysate (40 µg/Lane)
    Lane 6: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 43 kDa
    Observed band size: 45 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722334) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"