Apo-M Recombinant Rabbit Monoclonal Antibody [JE37-03]
Catalog# HA722333
Apo-M Recombinant Rabbit Monoclonal Antibody [JE37-03]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Apo-M Recombinant Rabbit Monoclonal Antibody [JE37-03]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Apo-M aa 1-100 / 188.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 21 kDa
Positive Control
Human plasma lysate, Mouse plasma lysate, Rat plasma lysate.
Conjugation
unconjugated
Clone Number
JE37-03
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Apolipoprotein M is an apolipoprotein and member of the lipocalin protein family that in humans is encoded by the APOM gene. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Two transcript variants encoding two different isoforms have been found for this gene, but only one of them has been fully characterized. It lacks an external amphipathic motif and is uniquely secreted to plasma without cleavage of its terminal signal peptide.
Background References
1. Xu T et al. ApoM suppresses kidney renal clear cell carcinoma growth and metastasis via the Hippo-YAP signaling pathway. Arch Biochem Biophys. 2023 Jul
2. Kazmirczak F et al. ApoM Activates Autophagy and Suppresses Lyosomal Lethargy to Combat Doxorubicin Cardiomyopathy. JACC Basic Transl Sci. 2023 Mar
Subcellular Location
Secreted.
Synonyms
Apo M antibody
Apo-M antibody
Apolipoprotein M antibody
ApoM antibody
APOM_HUMAN antibody
G3A antibody
HSPC336 antibody
MGC22400 antibody
NG20 antibody
NG20 like protein antibody
ExpandImages
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Western blot analysis of Apo-M on different lysates with Rabbit anti-Apo-M antibody (HA722333) at 1/1,000 dilution.
Lane 1: Human plasma lysate
Lane 2: Mouse plasma lysate
Lane 3: Rat plasma lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 21/25 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722333) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"