Mov10 Recombinant Rabbit Monoclonal Antibody [JE35-40]
Catalog# HA722328
Mov10 Recombinant Rabbit Monoclonal Antibody [JE35-40]
-
WB
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
Mov10 Recombinant Rabbit Monoclonal Antibody [JE35-40]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Mov10 aa 841-890 / 1,003.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 114 kDa
Positive Control
HEK-293 cell lysate, HeLa cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, rat liver tissue lysate, rat kidney tissue lysate.
Conjugation
unconjugated
Clone Number
JE35-40
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | IF-Cell | |
|---|---|---|---|
| human |
|
|
|
| mouse |
|
|
|
| rat |
|
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Putative helicase MOV-10 is an enzyme that in humans is encoded by the MOV10 gene. Stability of MOV10 protein is controlled via DCAF12 ubiquitin ligase.
Background References
1. Nawaz A et al. Unwinding the roles of RNA helicase MOV10. Wiley Interdiscip Rev RNA. 2022 Mar
2. Wang L et al. MOV10 Helicase Interacts with Coronavirus Nucleocapsid Protein and Has Antiviral Activity. mBio. 2021 Oct
Subcellular Location
Cytoplasm, P-body, Cytoplasmic ribonucleoprotein granule, Stress granule, Nucleus.
Synonyms
DKFZp667O1423 antibody
FLJ32791 antibody
fSAP113 antibody
Functional spliceosome-associated protein 113 antibody
gb110 antibody
MGC2948 antibody
Moloney leukemia virus 10 protein antibody
MOV 10 antibody
Mov10 (Moloney leukemia virus 10, mouse) homolog antibody
Mov10 (Moloney leukemia virus 10,) homolog antibody
ExpandImages
-
Western blot analysis of Mov10 on different lysates with Rabbit anti-Mov10 antibody (HA722328) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: RAW264.7 cell lysate (20 µg/Lane)
Lane 6: C6 cell lysate (20 µg/Lane)
Lane 6: Rat liver tissue lysate (40 µg/Lane)
Lane 6: Rat kidney tissue lysate (40 µg/Lane)
Predicted band size: 114 kDa
Observed band size: 114 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722328) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"