GUCY1B3 Recombinant Rabbit Monoclonal Antibody [JE41-09]
Catalog# HA722314
GUCY1B3 Recombinant Rabbit Monoclonal Antibody [JE41-09]
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WB
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
GUCY1B3 Recombinant Rabbit Monoclonal Antibody [JE41-09]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human GUCY1B3 aa 1-100 / 619.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 71 kDa
Positive Control
Human lung tissue lysate, human brain tissue lysate, mouse lung tissue lysate, mouse brain tissue lysate, mouse spleen tissue lysate, rat brain tissue lysate, rat spleen tissue lysate.
Conjugation
unconjugated
Clone Number
JE41-09
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Guanylate cyclase soluble subunit beta-1 is an enzyme that in humans is encoded by the GUCY1B3 gene. Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha and a beta subunit, catalyzes the conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide and nitrovasodilator drugs. GUCY1B3 has been shown to interact with Heat shock protein 90kDa alpha (cytosolic), member A1 and Endothelial NOS.
Background References
1. Korkmaz Y et al. Dental Pulp Inflammation Initiates the Occurrence of Mast Cells Expressing the alpha(1) and beta(1) Subunits of Soluble Guanylyl Cyclase. Int J Mol Sci. 2023 Jan
2. Vishnolia KK et al. sGC Activity and Regulation of Blood Flow in a Zebrafish Model System. Front Physiol. 2021 Feb
Subcellular Location
Cytoplasm.
Synonyms
GC-S-beta-1 antibody
GC-SB3 antibody
GCbeta1 antibody
GCS beta 1 antibody
GCS beta 3 antibody
GCS-beta-1 antibody
GCS-beta-3 antibody
GCYB1_HUMAN antibody
guanylate cyclase 1, soluble, beta 3 antibody
Guanylate cyclase soluble beta 1 chain antibody
ExpandImages
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Western blot analysis of GUCY1B3 on different lysates with Rabbit anti-GUCY1B3 antibody (HA722314) at 1/1,000 dilution.
Lane 1: Human lung tissue lysate
Lane 2: Human brain tissue lysate
Lane 3: Mouse lung tissue lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Mouse spleen tissue lysate
Lane 6: Rat brain tissue lysate
Lane 7: Rat spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722314) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"