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CARD11 Recombinant Rabbit Monoclonal Antibody [JE37-28]

Usd: 385

Specification

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Catalog# HA722309

CARD11 Recombinant Rabbit Monoclonal Antibody [JE37-28]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CARD11 Recombinant Rabbit Monoclonal Antibody [JE37-28]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Carma 1/CARD11 aa 1100-1154.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 133 kDa

Positive Control

K-562 cell lysate, HDLM-2 cell lysate, Raji cell lysate, mouse thymus tissue lysate, mouse spleen tissue lysate, rat thymus tissue lysate, human lymph node tissue, human spleen tissue, mouse thymus tissue.

Conjugation

unconjugated

Clone Number

JE37-28

Reactivity Data

WB IHC-P
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). CARD11 (CARMA1) has a domain structure similar to that of CARD10 (CARMA3) and CARD14 (CARMA2) as a member of the CARD-CC family with a C terminal MAGUK domain (the so-called CARMA proteins). The CARD domain of proteins in the CARD-CC family have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of NF-κB activation by recruitment and activation of MALT1. When overexpressed in cells, this protein family activates NF-κB and induces the phosphorylation of BCL10. CARD11 is critical for T cell and B cell function and is activated after T cell receptor or B cell receptor stimulation. After receptor stimulation, CARD11 is phosphorylated by PKC-θ (in T cells) or PKC-β (in B cells). The phosphorylation induces formation of filamentous CARD11 multimers that recruit BCL10 and MALT1, which in turn activates NF-κB. Loss of function mutations in CARD11 cause severe combined immunodeficiency (SCID) since the function of cells critical for adaptive immunity are disrupted.

Background References

1. Carter NM et al. CARD11 signaling in regulatory T cell development and function. Adv Biol Regul. 2022 May

2. Kameda T et al. CARD11 mutation and HBZ expression induce lymphoproliferative disease and adult T-cell leukemia/lymphoma. Commun Biol. 2022 Nov

Subcellular Location

Cytoplasm, Membrane raft.

UNIPROT

SwissProt: Q9BXL7 Human

SwissProt: Q8CIS0 Mouse

Entrez Gene: 304314 Rat

Synonyms

0610008L17Rik antibody

2410011D02Rik antibody

Bcl10 interacting maguk protein 3 antibody

BIMP 3 antibody

BIMP3 antibody

CAR11_HUMAN antibody

CARD 11 antibody

CARD containing MAGUK protein 3 antibody

Card maguk protein 1 antibody

CARD-containing MAGUK protein 1 antibody

Expand

Images

  • Western blot analysis of CARD11 on different lysates with Rabbit anti-CARD11 antibody (<a href="/products/HA722309" style="font-weight: bold;text-decoration: underline;">HA722309</a>) at 1/1,000 dilution.<br /><br />Lane 1: K-562 cell lysate (20 µg/Lane)<br />Lane 2: HDLM-2 cell lysate (20 µg/Lane)<br />Lane 3: Raji cell lysate (20 µg/Lane)<br />Lane 4: Mouse thymus tissue lysate (40 µg/Lane)<br />Lane 5: Mouse spleen tissue lysate (40 µg/Lane)<br />Lane 6: Rat thymus tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 133 kDa<br />Observed band size: 133 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722309" style="font-weight: bold;text-decoration: underline;">HA722309</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CARD11 on different lysates with Rabbit anti-CARD11 antibody (HA722309) at 1/1,000 dilution.

    Lane 1: K-562 cell lysate (20 µg/Lane)
    Lane 2: HDLM-2 cell lysate (20 µg/Lane)
    Lane 3: Raji cell lysate (20 µg/Lane)
    Lane 4: Mouse thymus tissue lysate (40 µg/Lane)
    Lane 5: Mouse spleen tissue lysate (40 µg/Lane)
    Lane 6: Rat thymus tissue lysate (40 µg/Lane)

    Predicted band size: 133 kDa
    Observed band size: 133 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722309) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of CARD11 on different lysates with Rabbit anti-CARD11 antibody (<a href="/products/HA722309" style="font-weight: bold;text-decoration: underline;">HA722309</a>) at 1/2,000 dilution. <br /><br />Lane 1: Jurkat cell lysate (10 µg/Lane) <br />Lane 2: Jurkat CARD11 KD cell lysate (10 µg/Lane) <br /><br />Predicted band size: 133 kDa <br />Observed band size: 133 kDa <br /><br />Exposure time: 24 seconds; ECL: K1802; <br /><br />4-20% SDS-PAGE gel. <br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722309" style="font-weight: bold;text-decoration: underline;">HA722309</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of CARD11 on different lysates with Rabbit anti-CARD11 antibody (HA722309) at 1/2,000 dilution.

    Lane 1: Jurkat cell lysate (10 µg/Lane)
    Lane 2: Jurkat CARD11 KD cell lysate (10 µg/Lane)

    Predicted band size: 133 kDa
    Observed band size: 133 kDa

    Exposure time: 24 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722309) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-CARD11 antibody (<a href="/products/HA722309" style="font-weight: bold;text-decoration: underline;">HA722309</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722309" style="font-weight: bold;text-decoration: underline;">HA722309</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-CARD11 antibody (HA722309) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722309) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CARD11 antibody (<a href="/products/HA722309" style="font-weight: bold;text-decoration: underline;">HA722309</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722309" style="font-weight: bold;text-decoration: underline;">HA722309</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CARD11 antibody (HA722309) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722309) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-CARD11 antibody (<a href="/products/HA722309" style="font-weight: bold;text-decoration: underline;">HA722309</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722309" style="font-weight: bold;text-decoration: underline;">HA722309</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-CARD11 antibody (HA722309) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722309) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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